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SC Spread ID	Images			Distance from centromere (percentage of arm length)
653-07 1	Composite	Fluorescence	Phase	66.9%
653-14 1	Composite	Fluorescence	Phase	67.3%
653-18 1	Composite	Fluorescence	Phase	69.0%
653-25 1	Composite	Fluorescence	Phase	68.2%
653-37 1	Composite	Fluorescence	Phase	71.3%
653-43 1	Composite	Fluorescence	Phase	66.6%
653-46 1	Composite	Fluorescence	Phase	72.3%
653-48 1	Composite	Fluorescence	Phase	66.4%
653-49 1	Composite	Fluorescence	Phase	71.7%
653-51 1	Composite	Fluorescence	Phase	73.0%
Mean±sd				69.3%±2.6%

Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 4.7µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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