Clone hba-49j9

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Clone
Clone name
hba-49j9
LE_HBa0049J09
C12HBa0049J09
P049J09
Clone type
BAC
Contamination
none
Estimated length
79,864
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
C2_At5g42740Tomato-EXPEN 2000plate 13E11
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 12
FISH map 
View on FISH map chromosome 12
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
653-07 1CompositeFluorescencePhase66.9%
653-14 1CompositeFluorescencePhase67.3%
653-18 1CompositeFluorescencePhase69.0%
653-25 1CompositeFluorescencePhase68.2%
653-37 1CompositeFluorescencePhase71.3%
653-43 1CompositeFluorescencePhase66.6%
653-46 1CompositeFluorescencePhase72.3%
653-48 1CompositeFluorescencePhase66.4%
653-49 1CompositeFluorescencePhase71.7%
653-51 1CompositeFluorescencePhase73.0%
Mean±sd69.3%±2.6%

Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 4.7µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
Italy
IL-mapped to chromosome
12
IL-mapped to IL segment
12-D
IL mapping notes
-
Sequencing  
This clone is being sequenced by the Chromosome 12 Sequencing Project. (View projects)
Log in as a curator or sequencer to edit this clone's registry information.
Sequencing Project
Chromosome 12
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'italy'
Full Sequence
98,089 bp
C12HBa0049J09.1
AC217250.1 (GenBank) 		[View]
End Sequences
Warnings
none
FTP
Chromosome 12 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (19)
1414, 1536, 1753, 1896, 2135, 2375, 2690, 2801, 3221, 3896, 3896, 4013, 4240, 5363, 5363, 5951, 7272, 9175, 10874
HindIII in silico (30)
1013, 1022, 1051, 1052, 1083, 1168, 1243, 1264, 1366, 1409, 1423, 1502, 1541, 1751, 1880, 2155, 2410, 2702, 2825, 3237, 3878, 3954, 4038, 4244, 5009, 5089, 5648, 7221, 9008, 10800
Fragment Lengths Match Score
0.78
Annotations  
Browse
LE_HBa0049J09 has no browsable sequence annotations
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temporarily unavailable
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 and  bp
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Annotations
End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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