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Dears friends: I want to Know if Are there microsatellite or other molecular marker that can be used for identify differents species in tomato??? i mean between sculentum and
Hi,
I am very confused browsing literature because I can not find a clear statement about the ploidy of N. benthamiana.
N. tabacum is clearly an allotetraploid, and tetraploid (2n = 4x = 48), with 4 copies of n=12. Genome size over 4.5 Gb, however,
N benthamiana it is reported to be an "ancient" allotetraploid, but currently it is n=19, and 38 total number of chromosomes, so it seems that from the ancient allotetraploid (48 chromosomes), genome fractionation has occurred and 38 remains, and that currently the genome is "diploid" (2n = 2x = 38). Is this correct?. Is N benthamiana diploid?.
Thank you very much.
Best regards,
Oncolytic viruses (OVs) are a class of viruses that can selectively replicate in and lyse cancer cells and subsequently spread with a tumor while not causing damage to normal cells. Besides the direct oncolytic activity, OVs are also effective in stimulating the body immune responses which can further help recogonize, control or destroy cancer cells. Through the combination of the tumor-specific cell lysis and immune stimulation, OVs act their antitumor activities in situ.

OVs encompass a broad diversity of DNA and RNA viruses, typically fall into 2 classes: 1) viruses that are naturally cancer-selective such as reovirus, 2) viruses that are genetically manipulated including measles virus, adenovirus, and herpes simplex virus (HSV-1), and so on.

To date, the effectiveness of OVs has been supported by many preclinical data. A few oncolytic viruses have been approved by Food and Drug Administration in the United States. The OV approach to cancer treatment is becoming more interesting for scientists. To see what Creative Biogene can do with oncolytic viruses, please visit the website at https://www.creative-biogene.com/products/oncolytic-virus.html.
This topic discusses issues in developing controlled-vocabularies for describing Solanaceae phenotypes and traits.
Most should be covered by the 'Solanaceae Phenotype Ontology' (SPO)
(see SGN->Tools->Ontology browser) which is growing by demand of its users community.
Terms which exist in other ontologies (PO, PATO, etc) are mapped to SPO terms.
In some cases it will be advised to use other vocabularies, instead of providing new SPO terms.
Biosafety testing is a series of assays to evaluate the potential biological risks of new biological products, including viral testing, microbial testing, cell, and molecular testing, and analytic testing. Health authorities such as the European Medicines Agency (EMA) and the US Food and Drug Administration (FDA) require all biopharmaceutical products and vaccines to undergo stringent testing to ensure the product is safe and free of contaminants.

As a leading biotechnology services and products supplier, Creative Biogene can offer clients a range of biosafety testing services and related assay kits. For more information, please click the link: https://www.creative-biogene.com/Services/Biosafety-Testing-Service
Genome Editing for targeted gene improvement is widely used in the field of plant science for basic research as well as for the specific improvement of desired traits in commercial crops. However, a major challenge in using CRISPR gene-editing technology for agricultural applications is that the target gene-edited crop plants need to be transgene-free to maintain trait stability and to gain regulatory approval for commercial production. How to achieve transgene-free genome editing in plants? Let's look at how Creative Biogene does that: https://www.creative-biogene.com/crispr-cas9/service/transgene-free-genome-editing-in-plants.html
Apoptosis, a form of programmed cell death, is a natural process for the removal of diseased, damaged, or unwanted cells such as those with potentially harmful mutations, aberrant substratum, or alterations in cell-cycle control. It plays a central role in proper embryonic development, immune system function, and hormone-dependent atrophy. The activation or inhibition of apoptosis has therapeutic value in cancer or HIV and neurodegenerative diseases.

Apoptosis can be triggered by two known pathways: the intrinsic pathway (also called the mitochondrial pathway) and the extrinsic pathway. The intrinsic pathway, as its name implies, is activated by intracellular stimuli such as irreparable genetic damage, hypoxia, extremely high concentrations of cytosolic Ca2+, and severe oxidative stress. This pathway is closely regulated by a group of proteins belonging to the Bcl-2 family and is the result of increased mitochondrial permeability and the release of pro-apoptotic molecules such as cytochrome c into the cytoplasm. The other pathway, extrinsic pathway is initiated when extracellular ligands binding to cell-surface death receptors. The best-known death receptors are the type 1 TNF receptor (TNFR1) and a related protein called Fas (CD95) and their ligands are called TNF and Fas ligand (FasL) respectively.

An understanding of the mechanism of apoptosis is important as it plays a pivotal role in the pathogenesis of many diseases. In some cases, the problem is due to too much apoptosis, such as in the case of degenerative diseases while in others, too little apoptosis is the culprit. Despite being the cause of the problem, apoptosis plays an important role in the treatment of cancer as it is a popular target of many treatment strategies. With years of development, Creative Biogene has the expertise and ability in offering a broad and comprehensive service in apoptosis biochemical screening and profiling.
Dear Sol genomics network,

Shin-Han Shiu, Marja Timmermans, and I are organizing this summer's Cold Spring Harbor "Frontiers and Techniques in Plant Sciences" (June 27 - July 17).

The course provides foundational work in plant sciences as well as practical laboratory experience in techniques like RNA-seq, proteomics, mapping-by-sequencing, genome editing, and imaging. In recent years the course has stressed the quantitative nature of plant biology and students will be introduced to computational techniques required for analyzing large datasets.

As you may know, this course has been going strong for >30 years and has been taken by many successful plant scientists. I encourage you to let students, postdocs and faculty colleagues know about the course, which will be taught be a fantastic group of investigators http://meetings.cshl.edu/courses/2014/c-plan14.shtml.

CSH can provide limited financial support for US students to cover their tuition room and board through an NSF grant and other sources of funding. Thus, I strongly encourage you to consider sending your new students and/or post-docs to the course, which has had a very strong positive impact on the trajectory of participants.

APPLICATIONS ARE DUE APRIL 15.

Thank you,

Mark Johnson
Brown University
Adenoviruses are a group of viruses that are non-enveloped, spherical, and 80-110 nm in size.

The genome of adenovirus is a linear, non-segmented double-stranded (ds) DNA that is between 26000 and 48000 bp containing 22-46 protein-coding genes. The genes are divided into the early phase (including E1, E2, E3, E4) and late phase (including L1, L2, L3, L4, and L5). Adenoviruses have a broad range of hosts and several adenoviral serotypes have been found, for example, there are 57 human adenovirus serotypes (HAdV-1 to 57) in seven species (Human adenovirus A to G).

Several features have made adenovirus an attractive vector in applications of research and gene therapy. These include 1) adenovirus can accommodate larger inserts, 2) adenovirus can be produced at high titers, 3) adenovirus can infect both dividing and non-dividing cells, 4) adenovirus do not integrate into cell genome, 5) adenovirus can mediate high transduction efficiency (almost 100%) in most cell types.

Creative Biogene is a biotechnology company that has years of experience working in the field of adenoviruses to improve the adenoviral genome's utility in research and its therapeutic potential. Please visit the website at https://www.creative-biogene.com/Services/Custom-Viral-Service/Adenovirus-service.html if you are interested.
Dear friends, I am about to begin an investigation of lycopene content in tomato species, as well as identification of microsatellites to identify the gene or genes responsible for the character under study, so I ask if I could help with information on the research topic . Thank you very much
Dear all,
I need your help here. Does anyone know how to convert ITAG gene tomato annotations to KEGG (or UniProt) ID?

Thanks in advance!

Irene
Started by:
Lukas Mueller
2021-10-17 21:26:26
 
In the next few years, hundreds of Solanaceae will be sequenced using next generation sequencing methods. If you intend to sequence a Solanaceae, please provide information on the species to avoid duplicate sequencing of accessions on the SGN SOL-100 page http://solgenomics.net/organism/sol100/view. This topic is for the discussion of SOL-100 related questions.
I would like to know whether Triticum aestivum database is updated and is there any reference where I researchers have designed construct for VIGS in wheat
The plant biotechnology and molecular breeding laboratory at Mid-Florida Research & Education Center at the University of Florida is seeking a highly motivated scientist to conduct studies of the environmental effect on tomato seed development, which is sponsored by FFAR for three years. The objective of this project is to identify the small RNAs that regulate seed development in response to maternal temperatures. This position requires a person with horticultural practices in growing tomato plants and a strong background in small RNA sequencing and analysis. The majority of research activities will be completed at Mid-Florida Education and Research Center, which is about 15miles from Orlando downtown, Florida; occasional travel to other states such as California may be required.

Applicants should receive a Ph.D. degree within the past 5 years with a solid background in bioinformatics and molecular biology/plant biotechnology, and have at least one first-authored publication in a peer-reviewed journal. Expertise in plant molecular techniques (small RNA delivery, siRNAs manipulation, CRISPR gene-editing) and research experiences in tomato or seed biology will be highly preferred. Candidates should have excellent skills in communications in oral and written English, skills in critical thinking, and are able to work independently in a culture-diverse environment. Applicants with legal visa status in the U.S. will be seriously considered. Salary is competitive and commensurate with background and experience($48000 plus medical benefit etc.). This position will be immediately available and remain open until filled. The term for this position is one year, and renewable contingent on academic performance for at least another 2 years. Qualified applicants should send a CV, and contact information for 3-4 references by email to hhuo@ufl.edu.
2021-10-01 05:19:15
 
Hi dears,
Is there any possibility that someone can share the cDNA library of benthamiana? Please let me know, if it is possible. Thanks.
Started by:
Douglas Maxwell
2021-09-30 12:51:34
 
Started by:
john binns
2021-09-16 06:14:04
 
This topic is for members of the SOL community from around the world to publish job openings. If interested, please reply directly to the poster. Job postings submitters please note that the postings will be removed by default 8 months after posting.
Started by:
Amir Tavassoli
2021-09-10 12:27:46
 
Dear Sir/Madam
Hi
I need all of tomato reference and integrated maps such as "Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589".
But when I choose the map, I see "Server error" message.
Please let me know how can I get these maps.
Regards.
Started by:
Sridhar Gutam
2021-09-08 11:37:17
 
Dear All,

I would like to work on phenotyping tomato and brinjal (egg plant) for abiotic stress tolerance traits. For that I would like to know what are the materials and methods needed.

Why I am asking is that if any one in the community had developed a set of protocols for low cost phenotyoing, i would like to use it.

Please connect me with people who are working on phenotyping tomato.

Thanks
Sridhar
Started by:
Peng Wang
2021-09-01 03:04:31
 
Here we report a serious problem with tobacco gene models. We tried to map the gene models of Nitociana tabacum (TN90), which you provide in your ftp, to the TN90 genome assembly sequences. Unfortunately, a lot of gene models could not be mapped. Of the 122388 gene models you provided in the gene model file,only 53714 could be mapped to the genome assembly. We do not know what method you used to identify gene models. Could you check all the datasets of the three tobacco varieties(TN90, K326 and Bathma)? Thank you.
Hello
I would like to access the potato annotation file in iTAG nomenclature with their respective TAIR nomenclature codes equivalent to each gene. Is that possible? I can not find the annotation file that includes that information. I need to match the nomenclature accepted in DAVID (The Database for Annotation).

Example in PGSC annotation available in Phytozome

PGSC0003DMG400000001 = AT1G12600.1
Is it safe to directly map the gene identifiers (Solyc00g005000.2) using the primary id (Solyc00g005000)?
Started by:
Lukas Mueller
2021-05-18 10:57:36
 
This topic contains release notes for new SGN data releases.
Hi, I would like to know if there is a straightforward way to translate the one nomenclature to the other.
Both refer to genes/loci/protein, right... but if I have to compare to see coincidences from one list in one format and one list in the other (having hundreds of proteins/genes in each list), I can die doing searches and endless alignments, and still not accomplishing the job...
Please help.
Thank you very much.
Started by:
Emilio Ortiz
2014-01-19 23:11:32
 
Hi,

Is there any way to download the 2860 COSII sequences? Please let me know.


Thank you,
Started by:
Eva Huala
2012-02-14 14:26:45
 
This topics is for the researchers of the SOL community around the world who need some specific materials for their experiments and express some request to those who could provide them with the expected materials or resources.
Started by:
Lukas Mueller
2009-07-23 12:10:22
 
This topic contains release notes for new SGN website features.
Started by:
Isaak Tecle
2008-06-03 15:53:50
 
Started by:
Isaak Tecle
2008-06-03 15:53:13