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SC Spread ID	Images			Distance from centromere (percentage of arm length)
348-06 1	Composite	Fluorescence	Phase	43.9%
348-14 1	Composite	Fluorescence	Phase	46.1%
359-15 1	Composite	Fluorescence	Phase	44.2%
359-18 1	Composite	Fluorescence	Phase	42.2%
348-22 1	Composite	Fluorescence	Phase	42.7%
348-23 1	Composite	Fluorescence	Phase	48.1%
348-24 1	Composite	Fluorescence	Phase	47.0%
348-26 1	Composite	Fluorescence	Phase	49.1%
359-29 1	Composite	Fluorescence	Phase	45.1%
359-40 1	Composite	Fluorescence	Phase	39.6%
Mean±sd				44.8%±2.9%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 5.9µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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