Clone hba-77o5

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Clone
Clone name
hba-77o5
LE_HBa0077O05
C04HBa0077O05
P077O05
Clone type
BAC
Contamination
none
Estimated length
118,522
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2390 : [coherent], 2 members:
C04HBa0077O05, LE_HBa0166O19
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1630Tomato-EXPEN 2000plate 7A11
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 4
FISH map 
View on FISH map chromosome 4
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
348-06 1CompositeFluorescencePhase43.9%
348-14 1CompositeFluorescencePhase46.1%
359-15 1CompositeFluorescencePhase44.2%
359-18 1CompositeFluorescencePhase42.2%
348-22 1CompositeFluorescencePhase42.7%
348-23 1CompositeFluorescencePhase48.1%
348-24 1CompositeFluorescencePhase47.0%
348-26 1CompositeFluorescencePhase49.1%
359-29 1CompositeFluorescencePhase45.1%
359-40 1CompositeFluorescencePhase39.6%
Mean±sd44.8%±2.9%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 5.9µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 4 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 4
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
131,149 bp
C04HBa0077O05.2
CU457751.8 (GenBank) 		[View]
End Sequences
Warnings
none
FTP
Chromosome 4 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (19)
2292, 2646, 3062, 3170, 3419, 3642, 3642, 3878, 3878, 4195, 5162, 5392, 5849, 8433, 8911, 10470, 13143, 13669, 13669
HindIII in silico (28)
1267, 1333, 1351, 1611, 1833, 1852, 1915, 2092, 2299, 2651, 3065, 3194, 3422, 3565, 3655, 3680, 3703, 3919, 3946, 4208, 4912, 5106, 5543, 8394, 8921, 10301, 12693, 13487
Fragment Lengths Match Score
0.79
Annotations  
Browse
LE_HBa0077O05 has no browsable sequence annotations
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temporarily unavailable
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 and  bp
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End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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