Clone Read LE_HBa0132M01_T7_99265

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0132M01_T7_99265
Primer
T7
ID from Seq. Facility
LE_HBa_132_M01-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
640 trimmed for vector and quality, 929 raw
Sequence
>LE_HBa0132M01_T7_99265 (raw untrimmed)
TAATCGGTTCCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCGTACTCAACCATGTTGTTTGTGCAGTTGAATCGTAGCTTAGCTGCCAT
AGGATAGTGCTGACCAGATTCTGATACTAGGACTGCTCCAACACCTTTACCTTGGTGATTCTCCGCTCCGTCAAAGAATAACCTCCAGCCTGGATATGCT
TCAGAAATATCTTCACCCACAAATGACACTTCTTCATCGTGAAAATATGTCTTAAGAGGTTCATACTCTTCGTCAATGAGATTTTCCGCAAGATGGTCAG
CCAAAGCTTGTACCTTTATTGCCTTCTGAGTCACATACACGATCAAACTCACTCAACAACATTTTCCATTTAGCTAACTTTCCGGTTGGCATCGCTTTCT
GGAAAATGTACTTCAACGGATCCATTCTGGAGATAAGATATGTACTATACGATGAAAAATAGTGTCTCAATTTCTGGGCAAGCCACGTCAGAGCACAACA
TGTTCTCTCTGGCAAAGTGCACCGAGACTCGTACGGAGTAAATTTCTTGCTTATGTAGTAGATAGCCTTTTCCTTCTTCCCTGTCTCGTCGTGTTGACCA
AGTACACATCCAAAGGCATTATCCAAACAGACAAATACAGCAACAAACGGCTCCCCTCTCGTGGAGGAACCAATACTGGTGGGGTTAAACATATAGCCCC
TTGATATCATCCAAAGTCGTTTGGCCCTCCTCAATCCACTTTGTTCTGGGTCTCTTTCTCTCCAATTTGTAAAACGGCTCCTCCACTCTCGTTGATTTTG
CTTTCCACCCTTTCAATTACTTTACCTTCCTCAAACCTCCATCCTTTCTTCCTTCGTTTTCGCTTGAGTATTTATGATTTACTTGAGTTGTGCTTATCTA
TTCTATCTACCTACTTTCTTACATTCTTA
>LE_HBa0132M01_T7_99265 (trimmed for quality and cloning vector)
AGCTTCGTACTCAACCATGTTGTTTGTGCAGTTGAATCGTAGCTTAGCTGCCATAGGATAGTGCTGACCAGATTCTGATACTAGGACTGCTCCAACACCT
TTACCTTGGTGATTCTCCGCTCCGTCAAAGAATAACCTCCAGCCTGGATATGCTTCAGAAATATCTTCACCCACAAATGACACTTCTTCATCGTGAAAAT
ATGTCTTAAGAGGTTCATACTCTTCGTCAATGAGATTTTCCGCAAGATGGTCAGCCAAAGCTTGTACCTTTATTGCCTTCTGAGTCACATACACGATCAA
ACTCACTCAACAACATTTTCCATTTAGCTAACTTTCCGGTTGGCATCGCTTTCTGGAAAATGTACTTCAACGGATCCATTCTGGAGATAAGATATGTACT
ATACGATGAAAAATAGTGTCTCAATTTCTGGGCAAGCCACGTCAGAGCACAACATGTTCTCTCTGGCAAAGTGCACCGAGACTCGTACGGAGTAAATTTC
TTGCTTATGTAGTAGATAGCCTTTTCCTTCTTCCCTGTCTCGTCGTGTTGACCAAGTACACATCCAAAGGCATTATCCAAACAGACAAATACAGCAACAA
ACGGCTCCCCTCTCGTGGAGGAACCAATACTGGTGGGGTT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.976072
Expected error %
1.34996
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT39963.1| Score 176 E Value 4e-43 Identity 39.30% Align. Length 537bp (83.91%) Frame 2
putative polyprotein [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU90285.1| Score 174 E Value 1e-42 Identity 43.90% Align. Length 639bp (99.84%) Frame 2
putative gag/pol polyprotein, 3'-partial [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0132M01
Clone
Clone name
hba-132m1
LE_HBa0132M01
P132M01
Clone type
BAC
Contamination
none
Estimated length
119,620
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found