Clone Read LE_HBa0132M01_SP6_99264

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0132M01_SP6_99264
Primer
SP6
ID from Seq. Facility
LE_HBa_132_M01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
1 trimmed for vector and quality, 1710 raw
Sequence
>LE_HBa0132M01_SP6_99264 (raw untrimmed)
GAATGATGCAGCACTATGAACGCTNGTAACGNCACGATCCTAGCGATCGCCTACCATAAGATACAACATACGGGAAGCGATGGCTGGCGAGTGCGATATG
AGAGACTGCGCATGAGAGGTTGTGTGGGCGATGGNTTGATCANCGNTCTGTGGACCGTGCTGGNATNGCGCTTCTGCAGTTCTATCAAGTCATNTGGATN
GCTGTAATCGTTCGTGCGCNTGACTACACACAGAGTGACACTATTAATGTNCTCACTCTGCAGGATACGCAGCTCTTAGCCGTGCTGGCACGCGCGTAAC
CGTGAGTCCAATGCTNCANGTGCAGNGCCTGATTGATTACTGCACTGCAGTCTGGAGATCATGGATGTACAATACGNAAGTTAACTTGAACGTCTNACGT
CTGACGTCATACAGCTTGTATGCTCTTACTGAGAACCGAAAGGCGGTGAGTCTGTAGCATNGTATGTACCGTGCTATANCGATATGGGATGTAGCCTCTG
TCGATGCTTCGGAGCACTAGCTCAGANATATACGCCGTGGAGCCTCTCTAGCGTGTGATCGTCGTGCGAGTTCGGATGGTATGATGCTAGCGCTGTGACA
TTGTACTTAACCGTTNACTTATCATGTGCGTTATTCCTCTGTCNGACACGCTTGGCGCCGCTGCGGCGCGTNGACTGCCTACTACGCTGCTCACTCCCTG
CGCTCCTTCCCTCCNACGCGACGCAGTANCTCGCGAGCGCCGCGCGAATGCGCGCGAACTGTCAGANTATCAGCGCNATTGANGTCGTGTCGTTGATGCG
CTGTGGTCGGGACTTGACACGCGCGCGATACAGTTCTCGTTAACGTCTGCTGTGCGTGTTTNTGTCGACCTGGCATCATNGTGATTTTTCGCGTTAGGCA
GGGCGCTGATGCGTGCGTCAGTGCGGCGCTAGTGCGAGATATACTGTGACACGCTNCTACTGACATNANGTAGANANATAGAATGTGATGATAGAGCATC
GAGATTGATGACTNGGGGATGTGTATGATGTGCACTGCGTCACACGNGAATCTGATCGCTTACGGAGCTTACGTAAACGAGACGCGCGACGATGGCTGAT
GCTAACTGGTNGCTGTTACATCCACTGCGCACCGCAGCGACTCNTGCGTGNAAGGNGTGNCTCGAATGTTGACGTGCGTGCGTGTGATGTACGGNCACAA
TGTTNATTCTNGNAGATATGCTGCTANTGGGACATGCGGTCCGTCTGGCTGCTGTGCATCNCACTGTGNCATGNCTCAGATGCGGTGAAGCTCCTGGTCG
CACGTAGTCTCATGAAACCCGTATCGCGCATCGCCTATCGGTATGAGTCCGCGCGTGTGTTGATGCGCGTATGGTTCATATCTNATGATACATATGCGAT
GTTAATGTATCAGTGTATTGGTTGTATGTGATTGTGAANGATGTCNTCATGAGAGTTGCGTGTATGAGTGATCGACAAGCTTCTAGACGTATACAGGTCT
GCTCCTTGACTCAGGATCATCTATGTATCTTTTAAGTGTTTGTGNCCTGTATCACGCTCTCTTCAANCCTCTGTATCTGCATCTGTTATAGCAGACACCT
GATCGCTCATGCTTGTGATTTGCGCTTCTCAGATCTGTATGTATTCTTGCTATTGGACTTCTGCTGGCACCACTACTTCTCCGTCAGCTGCTCACGTCTT
CTACATCGNC
>LE_HBa0132M01_SP6_99264 (trimmed for quality and cloning vector)
G

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
  • High predicted error rate
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
100
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0132M01
Clone
Clone name
hba-132m1
LE_HBa0132M01
P132M01
Clone type
BAC
Contamination
none
Estimated length
119,620
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found