Clone Read LE_HBa0069B03_T7_83381

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0069B03_T7_83381
Primer
T7
ID from Seq. Facility
LE_HBa_069_B03-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
452 trimmed for vector and quality, 876 raw
Sequence
>LE_HBa0069B03_T7_83381 (raw untrimmed)
CTCTACTTGGCCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCGATTCTTTCAGCAATTTTCCCCTTTTTGAGCCTTTTCAGCACTTCTG
ATATCATTTTTCTCCCTTATATTATTGGCTTTGAAGCTGCTTCGAGTCGCGCAGCAATGTTGTTGAGTGTAAATATTTTCAGCGCCTTTGTCATTATGAT
GGGGTAATGTTGATCCGTGTGAGTACATATCTCAGAAAATCCATCATGAGCTTGTTGAAATAAGGCCCCTAGTTGTGTCGATATCGTGTTCTCAGGGAAA
ACCTCCTCCAATTTCACCAGAAATAGAGATACACATACAAGTCGAGTTCTAGCATCGTCCAATTCATCAATTATGAAACTATCCTGTTAAGATAGCTTAT
AAACTAAACATGTATTCTTTTAATATTTCAGTTAGTTAGTTAGTTAGTTAGGTAATTAATTAGTTGGTTAAATACAGGTTAGTAAAGGTAGTTTCCTTCA
CACATAAATGTAATTTTATATATAATTCACATGTCCTCCATGTACATATATTCTCCGAACTCTATTCCGACTCTTTCTCCTTCTCCTCTCAATTAATCCT
CAATACTTCTTCAACTTCTTTAATCTTAAAGAAAAGCTTTTTTCCTTCTTTTCTTAATGTTCTTTTCTTTAGCATGTCTATGGCTTCTATTATTGTACTA
ATCTATTGTTTCCTATTGTTTATTTACCATCATCTCCTCCTATCCAATCTCACTCCCGTTGTCTTTCTTTCTCTTCTATTTGTATCATTTACCTCTTATC
TTTCTATGTACCCCTTTTCACTCTTCCACTCATTTAGCTACTGATGATTGTCTTCGTCTATTTTTTTTTTTCCTCT
>LE_HBa0069B03_T7_83381 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTCGATTCTTTCAGCAATTTTCCCCTTTTTGAGCCTTTTCAGCACTTCTGATATCATTTTTCTCCCTTATAT
TATTGGCTTTGAAGCTGCTTCGAGTCGCGCAGCAATGTTGTTGAGTGTAAATATTTTCAGCGCCTTTGTCATTATGATGGGGTAATGTTGATCCGTGTGA
GTACATATCTCAGAAAATCCATCATGAGCTTGTTGAAATAAGGCCCCTAGTTGTGTCGATATCGTGTTCTCAGGGAAAACCTCCTCCAATTTCACCAGAA
ATAGAGATACACATACAAGTCGAGTTCTAGCATCGTCCAATTCATCAATTATGAAACTATCCTGTTAAGATAGCTTATAAACTAAACATGTATTCTTTTA
ATATTTCAGTTAGTTAGTTAGTTAGTTAGGTAATTAATTAGTTGGTTAAATA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.96099
Expected error %
0.254414
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M16728.1:1-87 Score 62 E Value 5e-11 Identity 100.00% Align. Length 30bp (6.64%) Frame 2
S.aureus multiple cloning site vector upstream of beta-lactamase gene, clone pWN1818
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L24367.1:218-930 Score 62 E Value 5e-11 Identity 100.00% Align. Length 30bp (6.64%) Frame 2
Cloning vector pTugAS (derived from E. coli JM105) beta-lactamase and repressor protein gene, complete cds
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0069B03
Clone
Clone name
hba-69b3
LE_HBa0069B03
P069B03
Clone type
BAC
Contamination
none
Estimated length
133,366
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found