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Clone Read LE_HBa0040C21_T7_8201

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0040C21_T7_8201
Primer
T7
ID from Seq. Facility
LE_HBa_040_C21-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
760 trimmed for vector and quality, 936 raw
Genbank Accession
DU027440
Sequence
>LE_HBa0040C21_T7_8201 (raw untrimmed)
TAAACTGGGTACCGTGGGGTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTCTCATTGACATTTTCTTGTATAAGAGGTGCAGAAACATAGCATGTG
GCAGTGTGCTACCAGCAGAGGAGTGTAGCTGCGAAATTTGCTCGAAGAAAAATGGTTTCTGCAACCTTTGCATGTGTGCTATTTGTAACAAGTTTGATTT
TGAGGTCAACACTTGCAGATGGATTGGTTGTGATCTCTGTTCTCATTGGACACATACAGATTGTGCCATTAGCAATGGGCAGATTGGAACGGGCCCTTCT
GTTAAGGTTGGAGTTAGCTCAGCCGAGACGCTCTTCAGATGCCATGCTTGCAGTAGGACATCTGAGTTATTAGGTTGGGTTAAAGATGTGTTCCAGCACT
GTGCTCCAACTTGGGATGCTGAAGCTTTTGTTAGAGAGCTCGATTTCGTCAGGAGAATTTTCCAGAGAAGTGAGGACGCCAGAGGCCGCAAGTTATATTG
GAAATGCGAGGAACTCGTTGAAAAGCTGAAAGGTGGTGTTGCAGACCCAATGGCTTGTAAAGTGATCTTATCCTTTTTCCAAGGTAAGCCTGACATCTTA
GTTATTGTCCTAACTTTTTTCTACACTATTTGTGATTGGGGTGTAGTTGATAAATGATTGATTATTCTGGACCACACTTGAATTGGTCTGTCCGAGATTG
AGAAAAGGTTGAAATGAAAGCTTGATGCATCTAACAAGAAATCAAATGTAGCAGGTCACAATTTGATTATTGGTCCTCTGCTCTCTCTCTGGCTTTTTTG
ATCTATAGTTAGTGTTCTTTAGTAGGCTAGGTTGATTAAAAAGGTGTATAATAACAGTGGCGGACCCAAAAATTTCTAAGGGGTGTCACAATAAAAAAGA
ATAGAAACATGGAAAAAATAAAAGGTGGCAACCTTT
>LE_HBa0040C21_T7_8201 (trimmed for quality and cloning vector)
AGCTTCTCTCATTGACATTTTCTTGTATAAGAGGTGCAGAAACATAGCATGTGGCAGTGTGCTACCAGCAGAGGAGTGTAGCTGCGAAATTTGCTCGAAG
AAAAATGGTTTCTGCAACCTTTGCATGTGTGCTATTTGTAACAAGTTTGATTTTGAGGTCAACACTTGCAGATGGATTGGTTGTGATCTCTGTTCTCATT
GGACACATACAGATTGTGCCATTAGCAATGGGCAGATTGGAACGGGCCCTTCTGTTAAGGTTGGAGTTAGCTCAGCCGAGACGCTCTTCAGATGCCATGC
TTGCAGTAGGACATCTGAGTTATTAGGTTGGGTTAAAGATGTGTTCCAGCACTGTGCTCCAACTTGGGATGCTGAAGCTTTTGTTAGAGAGCTCGATTTC
GTCAGGAGAATTTTCCAGAGAAGTGAGGACGCCAGAGGCCGCAAGTTATATTGGAAATGCGAGGAACTCGTTGAAAAGCTGAAAGGTGGTGTTGCAGACC
CAATGGCTTGTAAAGTGATCTTATCCTTTTTCCAAGGTAAGCCTGACATCTTAGTTATTGTCCTAACTTTTTTCTACACTATTTGTGATTGGGGTGTAGT
TGATAAATGATTGATTATTCTGGACCACACTTGAATTGGTCTGTCCGAGATTGAGAAAAGGTTGAAATGAAAGCTTGATGCATCTAACAAGAAATCAAAT
GTAGCAGGTCACAATTTGATTATTGGTCCTCTGCTCTCTCTCTGGCTTTTTTGATCTATA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.966121
Expected error %
0.304692
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g48160.1 Score 288 E Value 2e-78 Identity 23.70% Align. Length 530bp (69.74%) Frame 3
68418.m05949 tropomyosin-related contains weak similarity to Tropomyosin, muscle (Allergen Ani s 3). (Swiss-Prot:Q9NAS5) [Anisakis simplex]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g07780.1 Score 285 E Value 3e-77 Identity 23.20% Align. Length 533bp (70.13%) Frame 3
68416.m00949 expressed protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP22954.1| Score 367 E Value 1e-100 Identity 31.10% Align. Length 533bp (70.13%) Frame 3
Potyvirus VPg interacting protein [Nicotiana benthamiana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAA96996.1| Score 288 E Value 8e-77 Identity 23.70% Align. Length 530bp (69.74%) Frame 3
unnamed protein product [Arabidopsis thaliana] ref|NP_199627.1| tropomyosin-related [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0040C21
Clone
Clone name
hba-40c21
LE_HBa0040C21
C05HBa0040C21
P040C21
Clone type
BAC
Contamination
none
Estimated length
100,590
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found