Clone Read LE_HBa0166C03_SP6_78052

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0166C03_SP6_78052
Primer
SP6
ID from Seq. Facility
LE_HBa_166_C03-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
664 trimmed for vector and quality, 834 raw
Sequence
>LE_HBa0166C03_SP6_78052 (raw untrimmed)
GGTTGTGGTTACTATGGCTTCTTCCAATTGCGAATCACGGAATTCCTTTGGATTTGTTGAGCAGAATCATCCGTTATGTACAAGACTTTCTTTGCTTGTT
CAGATGAAGAAAAACTATCGGTACCTTCTGATAACTATTTCAAAAGCACAAAGAAATCTGCTGGGACTTATGAGCAGCTACTGTTTCATCTTTCATCCTC
TGGTTTCAACGTCTGCCCCGAAAATCCACCTCGTTTCAAGTAATGTTTTACTAATTTATTGGAAACTGTTTCTAGTTTAAGCGATTTCCTGTTATGGTGA
TTTGAGAATGTTGTTTGTGAAATAAATCTGTTTTGATTTTGTTAGGCAAGTATTGGAGGAGATGGCTTCACATTTCACAAATTTAGGATTTGTATTGGGG
AGAATCATAAGTGAGTGTCTGGGCATCCCTCCTAACTTCCTAGCAAACTACAGAAATGATCAGGCTAAGGACTTCTTGCTCGGCATCCACTACAGTCCAG
CGACAGAAGCTGAGAACGTAGGAAAATCCGCACACAAAGATCCTGGCTGCATCACCATTCTCTACCAGCCTGAAGTTGGAGGTCTTCAGGTGCAGAAAGA
TGAACAGTGGATTCCCATAGCGCCTTCCAAAGACAAACTAGTAGTTAATATTGGTGATGTCATTCAGGTTAATTTATAGTTTGGTTATTTCTGATTTATT
TTCACTGTTATCAATTCTTCATTTAAACATTTTATTTTTGTATTTACGTTTTATAAAGGTGCTTAACCAACATTAAATTCAAGAATTGCAACGCTACGTG
TGTAACTGTACTTTGTGTAATTTTTATGTTTTCC
>LE_HBa0166C03_SP6_78052 (trimmed for quality and cloning vector)
CTTCCAATTGCGAATCACGGAATTCCTTTGGATTTGTTGAGCAGAATCATCCGTTATGTACAAGACTTTCTTTGCTTGTTCAGATGAAGAAAAACTATCG
GTACCTTCTGATAACTATTTCAAAAGCACAAAGAAATCTGCTGGGACTTATGAGCAGCTACTGTTTCATCTTTCATCCTCTGGTTTCAACGTCTGCCCCG
AAAATCCACCTCGTTTCAAGTAATGTTTTACTAATTTATTGGAAACTGTTTCTAGTTTAAGCGATTTCCTGTTATGGTGATTTGAGAATGTTGTTTGTGA
AATAAATCTGTTTTGATTTTGTTAGGCAAGTATTGGAGGAGATGGCTTCACATTTCACAAATTTAGGATTTGTATTGGGGAGAATCATAAGTGAGTGTCT
GGGCATCCCTCCTAACTTCCTAGCAAACTACAGAAATGATCAGGCTAAGGACTTCTTGCTCGGCATCCACTACAGTCCAGCGACAGAAGCTGAGAACGTA
GGAAAATCCGCACACAAAGATCCTGGCTGCATCACCATTCTCTACCAGCCTGAAGTTGGAGGTCTTCAGGTGCAGAAAGATGAACAGTGGATTCCCATAG
CGCCTTCCAAAGACAAACTAGTAGTTAATATTGGTGATGTCATTCAGGTTAATTTATAGTTTGG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.972086
Expected error %
0.32445
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g19000.2 Score 86 E Value 2e-17 Identity 14.50% Align. Length 338bp (50.90%) Frame 1
68416.m02412 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to SP|P24397 Hyoscyamine 6-dioxygenase (EC 1.14.11.11) (Hyoscyamine 6-beta- hydroxylase) {Hyoscyamus niger}, SP|Q05965 Naringenin,2-oxoglutarate 3-dioxygenase (EC 1.14.11.9) (Flavonone- 3-hydroxylase) (F3H) (FHT) {Matthiola incana}; contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g19000.1 Score 82 E Value 3e-16 Identity 14.40% Align. Length 326bp (49.10%) Frame 1
68416.m02411 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to SP|P24397 Hyoscyamine 6-dioxygenase (EC 1.14.11.11) (Hyoscyamine 6-beta- hydroxylase) {Hyoscyamus niger}, SP|Q05965 Naringenin,2-oxoglutarate 3-dioxygenase (EC 1.14.11.9) (Flavonone- 3-hydroxylase) (F3H) (FHT) {Matthiola incana}; contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_474083.1| Score 114 E Value 2e-24 Identity 15.60% Align. Length 326bp (49.10%) Frame 1
OSJNBa0063C18.20 [Oryza sativa (japonica cultivar-group)] emb|CAE05979.2| OSJNBa0063C18.20 [Oryza sativa (japonica cultivar-group)] emb|CAD41905.2| OSJNBa0033G05.6 [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB64346.1| Score 110 E Value 2e-23 Identity 14.40% Align. Length 326bp (49.10%) Frame 1
gibberellin 7-oxidase [Cucurbita maxima] pir||T09683 gibberellin 7-oxidase (EC 1.14.11.-) - winter squash
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0166C03
Clone
Clone name
hba-166c3
LE_HBa0166C03
P166C03
Clone type
BAC
Contamination
none
Estimated length
126,307
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found