Clone Read LE_HBa0153F12_T7_74375

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0153F12_T7_74375
Primer
T7
ID from Seq. Facility
LE_HBa_153_F12-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
708 trimmed for vector and quality, 847 raw
Genbank Accession
DU066540
Sequence
>LE_HBa0153F12_T7_74375 (raw untrimmed)
AGGCTCGGACCGGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGAACAGGTTGCTAGATGATAACAGAGAGCTTTTTGTCCCTGAGTTGTGT
GAAACTGTTCGGGCACACCCAAACTTCATGCTGTTTGCCACTCAAAATCCACCTACCTTATATGGTGGACGCAAAATTCTCTCACGAGCATTTCGCAACC
GTTTCGTGGAGATCCATGTCGATGAAATTCCAGAAGATGAGTTGAGCACAATATTGACAAATAGGTGTGAAATTCCAGAAAGCTATTCTAGGAAAATGAT
TGCTGTCATGAAGGAACTGCAATTGCATAGGCAGAGTACTAAAATTTTTGCTGGAAAACATGGTTTCATTACTCCACGCGATCTGTTTCGTTGGGCTAAT
CGTTTCAGGGAATTTGGGAAGTCCTACGAAGACCTAGCTCGTGATGGTTATTATTTGATGGCTGAGAGACTGCGAGATAATGACGAGAAAAAAGTTGTCC
AGGCAGTTTTGGAACAGCAGTTGCGGGTTAGACTTGCTGAAGATGATATGTACAAGCAGGTTTATCTCTTTTTCCTCACAACATACCTTGCGTATTACAT
AGTTATAAGTTTTTCTCTTATGGTTGTTATTGATGCTGGGAGATTCTCTGATTTTATGGAGTTACCATTTTTACCTCCCTTGGTTTTTCTAAATATATTG
CAAGGAATCTCAAAATGAAATAATTGTTTTTGCTTGGATACCACTTTAAATGAAGGAAAAATCAATTTTCAGCTCATCCTTTGTTGGAGCATTTGATTCT
TTCTCCTAAAAGGAAATTATTTCCCCTTTTTTTTTCTTTTATTAGGA
>LE_HBa0153F12_T7_74375 (trimmed for quality and cloning vector)
AGCTTTGAACAGGTTGCTAGATGATAACAGAGAGCTTTTTGTCCCTGAGTTGTGTGAAACTGTTCGGGCACACCCAAACTTCATGCTGTTTGCCACTCAA
AATCCACCTACCTTATATGGTGGACGCAAAATTCTCTCACGAGCATTTCGCAACCGTTTCGTGGAGATCCATGTCGATGAAATTCCAGAAGATGAGTTGA
GCACAATATTGACAAATAGGTGTGAAATTCCAGAAAGCTATTCTAGGAAAATGATTGCTGTCATGAAGGAACTGCAATTGCATAGGCAGAGTACTAAAAT
TTTTGCTGGAAAACATGGTTTCATTACTCCACGCGATCTGTTTCGTTGGGCTAATCGTTTCAGGGAATTTGGGAAGTCCTACGAAGACCTAGCTCGTGAT
GGTTATTATTTGATGGCTGAGAGACTGCGAGATAATGACGAGAAAAAAGTTGTCCAGGCAGTTTTGGAACAGCAGTTGCGGGTTAGACTTGCTGAAGATG
ATATGTACAAGCAGGTTTATCTCTTTTTCCTCACAACATACCTTGCGTATTACATAGTTATAAGTTTTTCTCTTATGGTTGTTATTGATGCTGGGAGATT
CTCTGATTTTATGGAGTTACCATTTTTACCTCCCTTGGTTTTTCTAAATATATTGCAAGGAATCTCAAAATGAAATAATTGTTTTTGCTTGGATACCACT
TTAAATGA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.970516
Expected error %
1.07206
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g67120.1 Score 265 E Value 1e-71 Identity 76.90% Align. Length 506bp (71.47%) Frame 3
68414.m07636 midasin-related similar to Midasin (MIDAS-containing protein) (Swiss-Prot:Q12019) [Saccharomyces cerevisiae]; similar to Midasin (MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo sapiens]; contains Prosite PS00017: ATP/GTP-binding site motif A (P-loop)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD10657.1| Score 265 E Value 6e-70 Identity 76.90% Align. Length 506bp (71.47%) Frame 3
Hypothetical protein [Arabidopsis thaliana] pir||B96695 hypothetical protein F5A8.4 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_176883.3| Score 265 E Value 6e-70 Identity 76.90% Align. Length 506bp (71.47%) Frame 3
midasin-related [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0153F12
Clone
Clone name
hba-153f12
LE_HBa0153F12
P153F12
Clone type
BAC
Contamination
none
Estimated length
89,971
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found