Clone Read LE_HBa0153F12_SP6_74374

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0153F12_SP6_74374
Primer
SP6
ID from Seq. Facility
LE_HBa_153_F12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
678 trimmed for vector and quality, 952 raw
Genbank Accession
DU066539
Sequence
>LE_HBa0153F12_SP6_74374 (raw untrimmed)
CCCGGCGATAGCTGTAGTAACAGGAAGAAGGGCTCAGTTGAATCTAGCAGAATGTGCTCTGATGTGGTTTCTGCCTGCATTTGCATGGGTTGGCTTGAAA
TTGCTCATGATATTTTGGATGATTTGGATTCTGAAGGAAATCCGCTGGACGCTAGTTCATACATGTCTCTGTTGACTGCATATTGCAACAGAAATAAGCT
AAGGGAGGCAGAGGCACTACTAAAGCAATTAAAAAGATCAGGTGTCATATTAGCATCTGATCCATTGTTAGCTCCTGCATCTATGTGTGAACTGGAAAGC
AAGAATAAGCTGAAAGAATTAGACACTTCGGCGAAAGGGGAGCTGGCTTACCATATTGTTGAAGAAATGAGAGCTGAAGAAAATGAAGCTTCTTTTATGA
TGCATGATTTAAATTTTTCAATCTACTTTTTTATGAAGGCTCACATGGTTGAAGATGCCGTAAGGGCTTACCGAAAAATGCAGGCAATGAAGATCCATCC
GACGGTGTCAACTTTCATGAATCTGCTTAATGGGTATTCTTCTTTAGGGATGTATCGTGAAATTACAATCTTGTGGGGAGATATTAAAAGGAACATGGAA
AGTCGTAAGAACTTGAATACCAGGGATTTATACGAGTTCTTGCTGTTGAATTTTCTTCGAGGCGGTTATTTTCATAGGGTGATGGAGGTTATTGGTTTTA
ATGAAAGAAAATGGCATGTATTTGGACAAGTGGATGTATAGACGTGAGTTCTTTGAAATATCACAAGGGTCTTTTCCTAAAAAATAAAAGTATCTGATGC
TAAAAATGAACTTCCAACTCCAAGGATTTGAGCTTGTGGGCCTTTTAGGAAAGGGGTTGGGCTGGATTAATTCTTACAGGTAATCAAATAAATTTTCTTT
GCTGCGGGAGAAAATTTCAAACACATCTCATGCCTCCTTTCCAAATTTTATA
>LE_HBa0153F12_SP6_74374 (trimmed for quality and cloning vector)
GCTCAGTTGAATCTAGCAGAATGTGCTCTGATGTGGTTTCTGCCTGCATTTGCATGGGTTGGCTTGAAATTGCTCATGATATTTTGGATGATTTGGATTC
TGAAGGAAATCCGCTGGACGCTAGTTCATACATGTCTCTGTTGACTGCATATTGCAACAGAAATAAGCTAAGGGAGGCAGAGGCACTACTAAAGCAATTA
AAAAGATCAGGTGTCATATTAGCATCTGATCCATTGTTAGCTCCTGCATCTATGTGTGAACTGGAAAGCAAGAATAAGCTGAAAGAATTAGACACTTCGG
CGAAAGGGGAGCTGGCTTACCATATTGTTGAAGAAATGAGAGCTGAAGAAAATGAAGCTTCTTTTATGATGCATGATTTAAATTTTTCAATCTACTTTTT
TATGAAGGCTCACATGGTTGAAGATGCCGTAAGGGCTTACCGAAAAATGCAGGCAATGAAGATCCATCCGACGGTGTCAACTTTCATGAATCTGCTTAAT
GGGTATTCTTCTTTAGGGATGTATCGTGAAATTACAATCTTGTGGGGAGATATTAAAAGGAACATGGAAAGTCGTAAGAACTTGAATACCAGGGATTTAT
ACGAGTTCTTGCTGTTGAATTTTCTTCGAGGCGGTTATTTTCATAGGGTGATGGAGGTTATTGGTTTTAATGAAAGAA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.952163
Expected error %
0.0556282
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g03100.1 Score 117 E Value 6e-27 Identity 11.10% Align. Length 656bp (96.76%) Frame 1
68414.m00285 pentatricopeptide (PPR) repeat-containing protein low similarity to CRP1 [Zea mays] GI:3289002; contains Pfam profile PF01535: PPR repeat
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g69290.1 Score 88 E Value 4e-18 Identity 9.80% Align. Length 629bp (92.77%) Frame 1
68414.m07946 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB10542.1| Score 200 E Value 3e-50 Identity 16.10% Align. Length 665bp (98.08%) Frame 1
hypothetical protein [Arabidopsis thaliana] emb|CAB78765.1| hypothetical protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_912237.1| Score 160 E Value 2e-38 Identity 14.00% Align. Length 641bp (94.54%) Frame 1
pentatricopeptide repeat-containing protein -like protein [Oryza sativa (japonica cultivar-group)] dbj|BAC21367.1| pentatricopeptide repeat-containing protein -like protein [Oryza sativa (japonica cultivar-group)] dbj|BAD30402.1| pentatricopeptide repeat-containing protein -like protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0153F12
Clone
Clone name
hba-153f12
LE_HBa0153F12
P153F12
Clone type
BAC
Contamination
none
Estimated length
89,971
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found