Clone Read LE_HBa0147E11_T7_72789

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0147E11_T7_72789
Primer
T7
ID from Seq. Facility
LE_HBa_147_E11-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
423 trimmed for vector and quality, 943 raw
Genbank Accession
DU065355
Sequence
>LE_HBa0147E11_T7_72789 (raw untrimmed)
GGGTTACCCTGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGCTGAGAAGTTCTGTAAAACTTAGTGAAATACAAATCTGTTAGGCG
ATGCGCCGAATGACCCTGAAGTCAAAATGTTGTCGCTGGATGGGAGAAAACTGGACAAATTGTAGGCCAACACACAAAAAGATAGCTGATGAATCATTTG
AGAACATTGATTGAAGATAGGCATATTTGATTGCTAAAAAAAGTTTTACATAGCTTAATAACTACTCGGTAGTGCACCCAACATACCACCATACCTTAAG
CTTAAGTCTACATCTGTTGAAGGTCTTAACATGAAGAAGCTACCTCAGCAGTATTATTCTCTATATCTATCTTTATGTTTCTTTGAGCTGACCTTCAGAT
TAATTTCATATATATTTGAAGACAAATAATTTTTTTAATGTGGTGGGGTGAGGCTCAATTGACTTATCCCATCTACGCCCCATTTGTTGCAGAACGGGCA
TTGTCAATGAGGGTGGTTACCCACGAACACTGGAGACTCTTTTCGCATGCATCACTACACATTCTGCGAACCCCCCCAATTGCATGTCCCACCATCTCCC
TCCAAGGACACTACATAATAAAGACTGGCGGCGGACAAAGAGGGATCTATGCTCTTCATTTTGGATAAGTATTTTATCTATCCTTCCCAATAATGGCCAC
TCTGTATCCTACTATTCCTATCTAATGTGTTTACTCTCTTTTATATCATAACTCACGGTTGGTAAAATATTGATTTGTGAATTCTCAAACTCTCGCTCGA
GACAGACTTTACTGTCTACATTCCTTTCCGATTGAATGACATTGTACTAATTCATAAGCGTACACCTTACGTTACACTCCTATTCAACCACCTTAGTATT
CAGCCGCTTTGAGGGGCGGTCGCAGTGGCGTGCAGTTTCTGGT
>LE_HBa0147E11_T7_72789 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTGCTGAGAAGTTCTGTAAAACTTAGTGAAATACAAATCTGTTAGGCGATGCGCCGAATGACCCTGAAGTCA
AAATGTTGTCGCTGGATGGGAGAAAACTGGACAAATTGTAGGCCAACACACAAAAAGATAGCTGATGAATCATTTGAGAACATTGATTGAAGATAGGCAT
ATTTGATTGCTAAAAAAAGTTTTACATAGCTTAATAACTACTCGGTAGTGCACCCAACATACCACCATACCTTAAGCTTAAGTCTACATCTGTTGAAGGT
CTTAACATGAAGAAGCTACCTCAGCAGTATTATTCTCTATATCTATCTTTATGTTTCTTTGAGCTGACCTTCAGATTAATTTCATATATATTTGAAGACA
AATAATTTTTTTAATGTGGTGGG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961516
Expected error %
0.526913
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L08951.1:26-123 Score 64 E Value 1e-11 Identity 100.00% Align. Length 31bp (7.33%) Frame 2
pT7T318 cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M62983.1:27-213 Score 64 E Value 1e-11 Identity 100.00% Align. Length 31bp (7.33%) Frame 2
Cloning vector M13JN72 containing mutliple cloning site
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0147E11
Clone
Clone name
hba-147e11
LE_HBa0147E11
C02HBa0147E11
P147E11
Clone type
BAC
Contamination
none
Estimated length
114,679
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found