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Clone Read LE_HBa0001N23_T7_669

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001N23_T7_669
Primer
T7
ID from Seq. Facility
Le-HBa001_N23-T7.ab1
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
771 trimmed for vector and quality, 1369 raw
Genbank Accession
DU021135
Sequence
>LE_HBa0001N23_T7_669 (raw untrimmed)
CTTGCCCGGGTCCGAGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTNGGGTTGCGCTGCATCATTTGGACTCTAGTTACAGAAAGCCAATGTG
AGCAAAAATATATACAAACTTTCTTACATTAGCCTCAAGGACTTCTCTGTGCAAGGTAATTTATTATTTAGATGCCGCTTTTTGAGCATTTTGCTGCCTT
CAAATCTACCAAGCTAACTTGGAATTTCTATTTTGTATCTCCATCTGTTCTGGGTTCTCTTCAAATGACTACAACAGTTGCACCCGAAGGTAATTTACCT
ACTTGTCTATTTCTTAATTCATCTGCATGCCCTGTGTTTTCTTCGGTCCTTTATTTATCACTAGAATTCGAAGCATCTGCTCTACGAAAGAAATATAATT
TTGAAACCCCCACCCCCCACTTCCTCTTAATGACTTACGTTGAAGTATCAGAAATTCAATCATGGGACGTGGAAACAGTTGGTTCAATCACATGATAATG
GATTAAAAGAATATTATTAGTCAATATTGCACTGGACGTATCATTGAAGATCAATGTAGTGGACAAAGGGGACAACTTTTCACTGTACAGTAGTCACAGT
GACTTCATGTCTGTAAAGTTTGGAAATTACACTGTCGTTTCATGCCTGAGAGAAGAAATCTACATTTTGTCTTGGAAAACATTGTTTTTGCCTTGGTATT
TATTACATGCATCAAATTGTCAAACTACTACATGTTAATTTTCTTCCTTTGGATGAGGTTTCTTGTGTGTTATATCATGTTTTTTGCCCATGTCAATTTC
TTCGTGATAGTTTTAGTTGATTAGTTTTTTTTTTCTTGCTCTTTTTGCGGGAGTAGTTTCTGGGTGTCTTTTGCCTAGTCTACCATCAAATGCTGGACTT
GACCAAAGTTCGGGGGATCACCTTCCTTTGGGATTTAAACGATAACTTTCAACCCCCTTGGCCATCTCTCTCTCAATTACCATTTTCCTCTGCAAAAAAA
GGATGTACAATTCCTTAATTTCTATTAAATCCAGTGCCCAAGTCCATTTTCTTGTAAAATAAACTCCTATTTTTTTTACTTACTTTTTGCTATTTGCGCC
GGTAAAATTTCTGGCCTTTCCTGCGGGAGCCATTTGGCCAGGCTTAAAAAAAAATTTTTACTTTTTTTCTTTAAACAAGTTTTAAAAAATACGCCGGTTT
TTTTTTTTTTGCCTTTCCATTAAAAAAAAATTTTTAAATTTTCTCCCGCGGAATAGGGAAAAAAAGGGGGGGGGGAAAAAAAAAAAAACCCGGGGGGAAA
ACCACAAAAAAAAAAGATTTTTTCTCAGATCTTTCCCACCCCACCACCAAATAATATTCTTGCGGTGGT
>LE_HBa0001N23_T7_669 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTTNGGGTTGCGCTGCATCATTTGGACTCTAGTTACAGAAAGCCAATGTGAGCAAAAATATATACAAACTT
TCTTACATTAGCCTCAAGGACTTCTCTGTGCAAGGTAATTTATTATTTAGATGCCGCTTTTTGAGCATTTTGCTGCCTTCAAATCTACCAAGCTAACTTG
GAATTTCTATTTTGTATCTCCATCTGTTCTGGGTTCTCTTCAAATGACTACAACAGTTGCACCCGAAGGTAATTTACCTACTTGTCTATTTCTTAATTCA
TCTGCATGCCCTGTGTTTTCTTCGGTCCTTTATTTATCACTAGAATTCGAAGCATCTGCTCTACGAAAGAAATATAATTTTGAAACCCCCACCCCCCACT
TCCTCTTAATGACTTACGTTGAAGTATCAGAAATTCAATCATGGGACGTGGAAACAGTTGGTTCAATCACATGATAATGGATTAAAAGAATATTATTAGT
CAATATTGCACTGGACGTATCATTGAAGATCAATGTAGTGGACAAAGGGGACAACTTTTCACTGTACAGTAGTCACAGTGACTTCATGTCTGTAAAGTTT
GGAAATTACACTGTCGTTTCATGCCTGAGAGAAGAAATCTACATTTTGTCTTGGAAAACATTGTTTTTGCCTTGGTATTTATTACATGCATCAAATTGTC
AAACTACTACATGTTAATTTTCTTCCTTTGGATGAGGTTTCTTGTGTGTTATATCATGTTTTTTGCCCATG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.965738
Expected error %
0.517668
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M19092.1:1-90 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.02%) Frame 2
Cloning vector pJRD179 DNA
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.02%) Frame 2
Cloning vector pMAMneoBlue
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001N23
Clone
Clone name
hba-1n23
LE_HBa0001N23
P001N23
Clone type
BAC
Contamination
none
Estimated length
101,571
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found