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Clone Read LE_HBa0001N23_SP6_668

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001N23_SP6_668
Primer
SP6
ID from Seq. Facility
Le-HBa001_N23-SP6.ab1
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
715 trimmed for vector and quality, 1241 raw
Genbank Accession
DU021134
Sequence
>LE_HBa0001N23_SP6_668 (raw untrimmed)
CCCAAAGTTTTAAGTACTTTGCCTTCCACTGCTTCCTGATGTGCACTATTTTCGCTTTAATCCAGGTGAAGTTACTTTACAATGTTGCCTGAGGTGAAAC
ATTATAAGCTAAAGATTGGTTCTTTCCTTTTGGGAAACCTTATTTCCCTAATTTTTTTCCTGAACTTTTGGCATTTCATAAACAATTCAATTTTTAGGTA
TCAGCCCGAATTAAAGAAAATGAATTAGGAGTATGATCTAGTCTTTCACTGCGGTGATATTGAGTTATAATTTTCTTCACATTGTTAACATGCTTTCTAC
ATATCTGGGATGCAGTTGATGAGCGATGTGATATGGAGCTAGATGAGACAGACCCTGCTGTCTGGTCAAAGTTAGAGGCTGCAACGGATGATTACATTCA
GAACACTTCTGCTGCTTTCAAGAATATTTGTGAAAGATTGTTGGAGAGGCCACATGATGAGAAATTTTCAGACAAAAAGTCCCATCAGTTTCTCAAAGCG
AAGAACTCTAAAACTGGTGTGTGAGGAATCATTTTTAATTTCTTGCCATGACATTCTTTTGTCTCAGATGGATTGAACTTTATTGGATTCAATTTATTTT
CAGATGAAAGCAGTCCTTCTTTAGGTTGGAGGAGGAGCGTGCTTCTTGTCGAGGCCCCCAAATAGTGCAGATGCTGGGAAGATTCTTTCACCATGTTCGC
TCACTAGAGTCATTGTGTGCTCGTTAATGGAAATAAAACTCAGTCTTTTTAAATGGCATATCAAAATACTCAGAAAGCAACTTCCAGGATCAACTTTTTC
CCGAACCCTTTTTTGCTTTCTCCTTTGTCCACTGGGGGATTTTCCCTCAAAGCCCCTCTTCTATACAGTCCCTGAAATGGGGGGCCTCATGGGGTTGGAA
GAATTTAATTAATTTCCACCCACTATACTTGTGAGGGATTTAAATTCTGCAAAAATCAACCGTCTTCCCCCCCTGATTCTCCCTCTAAAAAAATTGAAAG
CTTTTTCTTTCCCCCTTACAAACTCTTTGTATGAAAACCCTAAAAAACTTCCCTCTCAGGAGAGAAATAGGTGACCTCGGTCTCACGAAAAAGAAAAAGC
TTTCGGAGTTTTGGAGTCGAGGGGTGAGATTTATATCGTGTATCTTTTGTTTGATATCACCGGTAAATAGCGTCCTTTTTTAACTTCTTAATGTCCCCGT
AAAACCTCAAAACTATAAACATATGATTAAGTCATAAATAN
>LE_HBa0001N23_SP6_668 (trimmed for quality and cloning vector)
CACTGCTTCCTGATGTGCACTATTTTCGCTTTAATCCAGGTGAAGTTACTTTACAATGTTGCCTGAGGTGAAACATTATAAGCTAAAGATTGGTTCTTTC
CTTTTGGGAAACCTTATTTCCCTAATTTTTTTCCTGAACTTTTGGCATTTCATAAACAATTCAATTTTTAGGTATCAGCCCGAATTAAAGAAAATGAATT
AGGAGTATGATCTAGTCTTTCACTGCGGTGATATTGAGTTATAATTTTCTTCACATTGTTAACATGCTTTCTACATATCTGGGATGCAGTTGATGAGCGA
TGTGATATGGAGCTAGATGAGACAGACCCTGCTGTCTGGTCAAAGTTAGAGGCTGCAACGGATGATTACATTCAGAACACTTCTGCTGCTTTCAAGAATA
TTTGTGAAAGATTGTTGGAGAGGCCACATGATGAGAAATTTTCAGACAAAAAGTCCCATCAGTTTCTCAAAGCGAAGAACTCTAAAACTGGTGTGTGAGG
AATCATTTTTAATTTCTTGCCATGACATTCTTTTGTCTCAGATGGATTGAACTTTATTGGATTCAATTTATTTTCAGATGAAAGCAGTCCTTCTTTAGGT
TGGAGGAGGAGCGTGCTTCTTGTCGAGGCCCCCAAATAGTGCAGATGCTGGGAAGATTCTTTCACCATGTTCGCTCACTAGAGTCATTGTGTGCTCGTTA
ATGGAAATAAAACTC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.960337
Expected error %
0.342028
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g61850.1 Score 89 E Value 5e-19 Identity 43.60% Align. Length 415bp (58.04%) Frame 2
68414.m06979 patatin family protein similar to membrane-associated calcium-independent phospholipase A2 gamma; IPLA2 gamma [Homo sapiens] GI:8453174; contains Patatin domain PF01734, PF00514: Armadillo/beta-catenin-like repeat
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD30421.1| Score 92 E Value 1e-17 Identity 47.00% Align. Length 418bp (58.46%) Frame 2
putative calcium-independent phospholipase A2 [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_176378.1| Score 89 E Value 2e-17 Identity 43.60% Align. Length 415bp (58.04%) Frame 2
patatin family protein [Arabidopsis thaliana] gb|AAC28504.1| Contains similarity to gb|U51898 Ca2+-independent phospholipase A2 from Rattus norvegicus. [Arabidopsis thaliana] pir||T02131 hypothetical protein F8K4.6 - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001N23
Clone
Clone name
hba-1n23
LE_HBa0001N23
P001N23
Clone type
BAC
Contamination
none
Estimated length
101,571
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found