Clone Read LE_HBa0101P10_T7_57187

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0101P10_T7_57187
Primer
T7
ID from Seq. Facility
LE_HBa_101_P10-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
660 trimmed for vector and quality, 792 raw
Sequence
>LE_HBa0101P10_T7_57187 (raw untrimmed)
AACGCAGATTCTCCGCAGCCATGATGCCAGCTCGCCCGGCGTCATGGCGGCTATTTGTGCGCCGACATTAACCAGCGCCTGGGCCGTATCGCGGTCATAG
CAAGGTGTTGCGGTGCTATCGAGCGCTGCCAGTCCCAGCACTTTGATGCCGCTCTGGACACACTTTTTCACCTGATGCGTCAGTAATGATGATGAACCCC
CTTCGTAAAAATCGCTCACGAGGATAATGACGCTTTTCGCTGGTTGTTCAATAAGTTGCCGACCATACTCCACGGCACTGGCGATATTGGTCCCGCCGCC
CAACTGTACTTTCATTAATAACTCTACCGGATCGGCAACGTCTGCCGTGAGATCAACGACGCTTGTGTCAAACGCCACCAGATGGGTACGAATGCCGGGT
AACTGCCACAAACAGGCCGCCATCACCGCAGAGTGGATCACCGAATCGACCATCGATCCGCTTTGATCAACCAGTAAGACCAGTTGCCATTGTTCGCTTT
GGCGTTTAATGCGGCTGTTAAAGCGGGGGGATTCGACCGCCCACCCGCCGTGTTGCGGGTGCCAGTGTTGCAGGTTGGCGCGCAGAGTACTTTTGAAATC
AAAGTTTCGCGCCAGTGGAATAAATGAACGGCGACGGCGATCGCGGAAACCAGAAAAAGCCTGACGAACTTCCTTTGCCAGTCCAACCATAATTTCTTTC
ACAACCTGGCACACTATCCGGCGGGCGGGAGCCAGTACCTTCGGGTTCCTCAGATGTTTGGGTGTGCAAAATACCGTCGTATCAAGTTTTTA
>LE_HBa0101P10_T7_57187 (trimmed for quality and cloning vector)
CTCCGCAGCCATGATGCCAGCTCGCCCGGCGTCATGGCGGCTATTTGTGCGCCGACATTAACCAGCGCCTGGGCCGTATCGCGGTCATAGCAAGGTGTTG
CGGTGCTATCGAGCGCTGCCAGTCCCAGCACTTTGATGCCGCTCTGGACACACTTTTTCACCTGATGCGTCAGTAATGATGATGAACCCCCTTCGTAAAA
ATCGCTCACGAGGATAATGACGCTTTTCGCTGGTTGTTCAATAAGTTGCCGACCATACTCCACGGCACTGGCGATATTGGTCCCGCCGCCCAACTGTACT
TTCATTAATAACTCTACCGGATCGGCAACGTCTGCCGTGAGATCAACGACGCTTGTGTCAAACGCCACCAGATGGGTACGAATGCCGGGTAACTGCCACA
AACAGGCCGCCATCACCGCAGAGTGGATCACCGAATCGACCATCGATCCGCTTTGATCAACCAGTAAGACCAGTTGCCATTGTTCGCTTTGGCGTTTAAT
GCGGCTGTTAAAGCGGGGGGATTCGACCGCCCACCCGCCGTGTTGCGGGTGCCAGTGTTGCAGGTTGGCGCGCAGAGTACTTTTGAAATCAAAGTTTCGC
GCCAGTGGAATAAATGAACGGCGACGGCGATCGCGGAAACCAGAAAAAGCCTGACGAACT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.985009
Expected error %
0.510888
Sequence Annotations 
Automatic Annotations
E. coli K12 Genome (E.coli_K12/Ecoli_genome)NCBI BLAST
gi|16127994|ref|NC_000913.1| Score 1233 E Value 0 Identity 98.80% Align. Length 653bp (98.94%) Frame 2
Escherichia coli K12, complete genome
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_416625.1| Score 406 E Value 1e-112 Identity 31.80% Align. Length 653bp (98.94%) Frame 1
hypothetical protein b2121 [Escherichia coli K12] gb|AAC75182.1| orf, hypothetical protein; conserved protein [Escherichia coli K12] gb|AAA60484.1| yehP [Escherichia coli] pir||H64979 hypothetical protein yehP - Escherichia coli (strain K-12) sp|P33352|YEHP_ECOLI Hypothetical protein yehP prf||2014253Q yehP gene
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_708009.2| Score 403 E Value 1e-111 Identity 31.50% Align. Length 653bp (98.94%) Frame 1
hypothetical protein SF2190 [Shigella flexneri 2a str. 301] gb|AAN43716.2| orf, conserved hypothetical protein [Shigella flexneri 2a str. 301] ref|NP_837725.1| hypothetical protein S2315 [Shigella flexneri 2a str. 2457T] gb|AAP17534.1| hypothetical protein S2315 [Shigella flexneri 2a str. 2457T]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0101P10
Clone
Clone name
hba-101p10
LE_HBa0101P10
P101P10
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found