Clone Read LE_HBa0074N01_T7_55537

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0074N01_T7_55537
Primer
T7
ID from Seq. Facility
LE_HBa_074_N01-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
701 trimmed for vector and quality, 853 raw
Sequence
>LE_HBa0074N01_T7_55537 (raw untrimmed)
CTATACCTCGGTCCGCGGGCTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCATCGTCGAGAGGGAAACAGCCTAAATAACCAGCTAAGGCCCCTAAA
TGACCGCTCAGTGATAAAGGAGGTAGGGGTGCAGAGAAAGCCAGGAGGTTCCTAGAAGCAGCCACCATTGAAAGAGTGCGTAATAGCTCACTGATCGAGT
GCTCTTGCATCGAAGAATAACGGGGCTAAGCGGTCTGCCGAAGCTGTGGTATGTAAAAATACATCGGTAAGGGAGTGTTCCGCCTTAGAGAGAAGCCTGT
GTGTGAGCGGTGGTGGATGAAACAGAAGTGAAAATGTTGGCTTGACTAACGCAAAAATTGGTGAGAATCCAATGCCCCAAAAAACTAAGGGTTCCTCCGC
AAGGTTCGTCCACAGAAGGTGAGTCAGGGCCTAAGATAAGGCCGAAAGGCGTAGTTTATGGACAACAGGTGAATATTCCTGTACTACTTCTTGTTAGTCC
AAGGTACGGAGGAGGCTCGGTTAGCCGAAAGATGGTTATAGGTTCAAGAACGTAAGGTGTCCCTGCTTTTTCAGGGTAAGAAGGAGTAGAGAAAATGCCT
CGAGCCTATGTTCGAATACTAGGTGCTACGATGTTGAATTAACCCATGGCATACTCCCCCGAAATGCTCAAACCACGTTAAGCGAGGGTACCTGTACCCC
AAACTGACCCCGGTGGGTAGGGTAAGAATCCCTAGGGGGCCCGAGACCACACTCTCCAAGGAACTCCGCAAACTAGCCTGTAACTTCCGGAGAATGGGTG
CCTATTCAAAAGGGGGTTGCTATGACTGGGCTCGGGCCATGGTTTCCTAAATC
>LE_HBa0074N01_T7_55537 (trimmed for quality and cloning vector)
AGCTTCATCGTCGAGAGGGAAACAGCCTAAATAACCAGCTAAGGCCCCTAAATGACCGCTCAGTGATAAAGGAGGTAGGGGTGCAGAGAAAGCCAGGAGG
TTCCTAGAAGCAGCCACCATTGAAAGAGTGCGTAATAGCTCACTGATCGAGTGCTCTTGCATCGAAGAATAACGGGGCTAAGCGGTCTGCCGAAGCTGTG
GTATGTAAAAATACATCGGTAAGGGAGTGTTCCGCCTTAGAGAGAAGCCTGTGTGTGAGCGGTGGTGGATGAAACAGAAGTGAAAATGTTGGCTTGACTA
ACGCAAAAATTGGTGAGAATCCAATGCCCCAAAAAACTAAGGGTTCCTCCGCAAGGTTCGTCCACAGAAGGTGAGTCAGGGCCTAAGATAAGGCCGAAAG
GCGTAGTTTATGGACAACAGGTGAATATTCCTGTACTACTTCTTGTTAGTCCAAGGTACGGAGGAGGCTCGGTTAGCCGAAAGATGGTTATAGGTTCAAG
AACGTAAGGTGTCCCTGCTTTTTCAGGGTAAGAAGGAGTAGAGAAAATGCCTCGAGCCTATGTTCGAATACTAGGTGCTACGATGTTGAATTAACCCATG
GCATACTCCCCCGAAATGCTCAAACCACGTTAAGCGAGGGTACCTGTACCCCAAACTGACCCCGGTGGGTAGGGTAAGAATCCCTAGGGGGCCCGAGACC
A

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.974969
Expected error %
0.610117
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|ZP_00327143.1| Score 86 E Value 7e-16 Identity 22.90% Align. Length 191bp (27.25%) Frame 1
hypothetical protein Tery02002584 [Trichodesmium erythraeum IMS101]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAO74140.1| Score 77 E Value 3e-13 Identity 23.30% Align. Length 179bp (25.53%) Frame 1
ORF64c [Pinus koraiensis] ref|NP_817269.1| ORF64c [Pinus koraiensis]
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 153 E Value 2e-38 Identity 92.50% Align. Length 132bp (18.83%) Frame 2
No sequence description found.
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 779 E Value 0 Identity 90.10% Align. Length 689bp (98.29%) Frame 2
Nicotiana tabacum plastid, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0074N01
Clone
Clone name
hba-74n1
LE_HBa0074N01
C00HBa0074N01
P074N01
Clone type
BAC
Contamination
none
Estimated length
120,646
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found