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Clone Read LE_HBa0066J09_SP6_50752

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0066J09_SP6_50752
Primer
SP6
ID from Seq. Facility
LE_HBa_066_J09-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
656 trimmed for vector and quality, 936 raw
Sequence
>LE_HBa0066J09_SP6_50752 (raw untrimmed)
CCCGCACTACCCCCCTTCTGTTCCCATTCTTGATCAAGACAATCCAATGCAGTGCATTTATCAATTAGTTGAACTCTTGTATGTTTCTCTAATGATATCA
TTGACAGTTGTTCTCCAGCTACAGTTAATGTATATGGCTTAAGGTGTTTCTTTAAGATCCAAATGCAGAAATTTAATAAGATTTCTTGTCCAAATCTGCA
GAAAATCCGGTCTCACTCAACAGACATGTCACTCGTGCTATTAGATGAAGTATGTAATTCAGCAGTTGTTCATTTGTTCTTCCAGAGACACATGCTATGA
ACAAGTGAAATTATGCATGACATGAATGGACAGTTTCCTTCATAAAAACCATAGTGTGTATTCTTCATCTTGTAGGTAGGGGCAGGGACAAATCCTCTAG
AAGGAGCTGCACTTGGGATGTCATTACTGGAATCTTTTGCAGAATCTGGGACTTTGTTGACTATAGCTACAACACATCATGGAGAACTTAAAACCCTTAA
ATACAGGTGCTCAATGCTAAGATGGAACTTTTATATGAGACAAAAGGCTTACCTTCTCTAACATCATGGTGCTACAATGTACTTTACAGCAATCATGCAT
TTGAAAATGCATGTATGGAGTTCGATGAAATGAAGTTGAAACCAACTTTTAGGATTCTTTGGGGAATACCAGGTTGTCCTTGTATCCATGTGGTAGTTTC
TTGATTAGCCTGGAATTTCTTATATGACTGATCATAGGATGTCCATGTTCCTTCCTGCGGCAAGAAGAAGAGAAGAAATGATACTTCCTCCTTTGTCTTA
AAAAAAAAAAAATACCTCTTTACTCCCAAATCAATATTTCAGGGCCCAATCTCATGTCCCAAATTATTTTGAAATGCAGTTTTCCTAATTTTCATCCTTC
TAAGATCCTTTCCTTTTTTACCATTACCAATATTCC
>LE_HBa0066J09_SP6_50752 (trimmed for quality and cloning vector)
AATCCAATGCAGTGCATTTATCAATTAGTTGAACTCTTGTATGTTTCTCTAATGATATCATTGACAGTTGTTCTCCAGCTACAGTTAATGTATATGGCTT
AAGGTGTTTCTTTAAGATCCAAATGCAGAAATTTAATAAGATTTCTTGTCCAAATCTGCAGAAAATCCGGTCTCACTCAACAGACATGTCACTCGTGCTA
TTAGATGAAGTATGTAATTCAGCAGTTGTTCATTTGTTCTTCCAGAGACACATGCTATGAACAAGTGAAATTATGCATGACATGAATGGACAGTTTCCTT
CATAAAAACCATAGTGTGTATTCTTCATCTTGTAGGTAGGGGCAGGGACAAATCCTCTAGAAGGAGCTGCACTTGGGATGTCATTACTGGAATCTTTTGC
AGAATCTGGGACTTTGTTGACTATAGCTACAACACATCATGGAGAACTTAAAACCCTTAAATACAGGTGCTCAATGCTAAGATGGAACTTTTATATGAGA
CAAAAGGCTTACCTTCTCTAACATCATGGTGCTACAATGTACTTTACAGCAATCATGCATTTGAAAATGCATGTATGGAGTTCGATGAAATGAAGTTGAA
ACCAACTTTTAGGATTCTTTGGGGAATACCAGGTTGTCCTTGTATCCATGTGGTAG

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.955398
Expected error %
0.128847
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g54090.1 Score 84 E Value 1e-28 Identity 49.50% Align. Length 478bp (72.87%) Frame 1
68418.m06734 DNA mismatch repair MutS family protein low similarity to SP|Q56239 DNA mismatch repair protein mutS {Thermus aquaticus; contains Pfam profile PF00488: MutS domain V
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP54555.1| Score 85 E Value 1e-26 Identity 78.80% Align. Length 298bp (45.43%) Frame 1
putative DNA mismatch repair protein [Oryza sativa (japonica cultivar-group)] ref|NP_922268.1| putative DNA mismatch repair protein [Oryza sativa (japonica cultivar-group)] gb|AAM94926.1| putative DNA mismatch repair protein [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAA97131.1| Score 84 E Value 6e-27 Identity 49.50% Align. Length 478bp (72.87%) Frame 1
DNA mismatch repair protein MutS2-like [Arabidopsis thaliana] ref|NP_200220.1| DNA mismatch repair MutS family protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0066J09
Clone
Clone name
hba-66j9
LE_HBa0066J09
C03HBa0066J09
P066J09
Clone type
BAC
Contamination
none
Estimated length
115,324
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found