Clone Read LE_HBa0065J03_T7_49973

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0065J03_T7_49973
Primer
T7
ID from Seq. Facility
LE_HBa_065_J03-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
739 trimmed for vector and quality, 905 raw
Sequence
>LE_HBa0065J03_T7_49973 (raw untrimmed)
TTTAATTGGGGTCCCGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAACAATCATAGTAATGAATCCAGCTAGAAGCGCAGCCGCAATAATAC
ACCCCACAGTCCACTTAAAAATCTTCCTACTCACAATAATACAACCAAGATCCACATACTTCTTCTTCTTCTGCTCAGTCGGACCCAACAGCCAGCTCTG
TTGAGTCTCATCCAGTGACCTAGACATCGCTGCCCTATCAAAATCCTGCAAGTTCCTGCTCCTGTCATCGTCTGTAGCTGAATCAGCTGTGTGGATCTCT
AAAGGTCCACCCCATGGATCCCTACCGTACATATTGATTCTGGGTACTCAAATTAGAGCTGAAAAAACACCCCCACAAGCTCAAAACATGCAAAGGTTGA
ATCTTTTTTCCAAGATTCAAGAAATGCTGAACTGGGTAAGTCTTGATCTACTGTTTTTTTTTTTCCTTTTTTCAAGAAAGTTGTGGAATGAAAGGAGATG
GCAGAAAGGATGGGGTATTATGGAGGAAGGAGGAAAATGGGACTAGAAAATCTAGTGGAAAATAATGGATATATGAAAGCTGTGATGGTGCTGTTTTTTT
GGCTTTTTTGATTATAACACAATATATTTAATACTAGTAATATTACCAAACTTGAGAAAAAGAAAACAAAAGAGTGAAGTAATGCAGAAGAAAAATATAT
CAAAAAAAAAGGAAAGTAGTAGTAGTTGATGACACTGACAATACAGTAATGGACAATGACAACCTGTCCATTGCCAATCCCCCTCCTTTAATGGGGTGTT
TCACTTTCTTATTTCTTATTTACTTGTTAGGACAAAATCTCCTTAAATATAAAAAATTTCAAACTTACAATGGTTAAAATACACCTCCAATTTAAAATTT
GTGGT
>LE_HBa0065J03_T7_49973 (trimmed for quality and cloning vector)
AGCTTAACAATCATAGTAATGAATCCAGCTAGAAGCGCAGCCGCAATAATACACCCCACAGTCCACTTAAAAATCTTCCTACTCACAATAATACAACCAA
GATCCACATACTTCTTCTTCTTCTGCTCAGTCGGACCCAACAGCCAGCTCTGTTGAGTCTCATCCAGTGACCTAGACATCGCTGCCCTATCAAAATCCTG
CAAGTTCCTGCTCCTGTCATCGTCTGTAGCTGAATCAGCTGTGTGGATCTCTAAAGGTCCACCCCATGGATCCCTACCGTACATATTGATTCTGGGTACT
CAAATTAGAGCTGAAAAAACACCCCCACAAGCTCAAAACATGCAAAGGTTGAATCTTTTTTCCAAGATTCAAGAAATGCTGAACTGGGTAAGTCTTGATC
TACTGTTTTTTTTTTTCCTTTTTTCAAGAAAGTTGTGGAATGAAAGGAGATGGCAGAAAGGATGGGGTATTATGGAGGAAGGAGGAAAATGGGACTAGAA
AATCTAGTGGAAAATAATGGATATATGAAAGCTGTGATGGTGCTGTTTTTTTGGCTTTTTTGATTATAACACAATATATTTAATACTAGTAATATTACCA
AACTTGAGAAAAAGAAAACAAAAGAGTGAAGTAATGCAGAAGAAAAATATATCAAAAAAAAAGGAAAGTAGTAGTAGTTGATGACACTGACAATACAGTA
ATGGACAATGACAACCTGTCCATTGCCAATCCCCCTCCT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.961094
Expected error %
0.712201
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g49720.1 Score 176 E Value 1e-44 Identity 29.80% Align. Length 281bp (38.02%) Frame 1
68418.m06157 endo-1,4-beta-glucanase KORRIGAN (KOR) / cellulase (OR16pep) identical to endo-1,4-beta-D-glucanase KORRIGAN [Arabidopsis thaliana] GI:3978258; similar to endo-1,4-beta-D-glucanase; cellulase GI:5689613 from [Brassica napus]; identical to cDNA cellulase (OR16pep) GI:1022806
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g24260.1 Score 158 E Value 3e-39 Identity 26.60% Align. Length 281bp (38.02%) Frame 1
68417.m03481 endo-1,4-beta-glucanase, putative / cellulase, putative similar to endo-1,4-beta-D-glucanase; cellulase GI:5689613 from [Brassica napus]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC49704.1| Score 195 E Value 1e-48 Identity 33.30% Align. Length 281bp (38.02%) Frame 1
endo-1,4-beta-glucanase [Lycopersicon esculentum] pir||T07612 cellulase (EC 3.2.1.4) Cel3, membrane-anchored - tomato
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB51903.1| Score 176 E Value 6e-43 Identity 29.80% Align. Length 281bp (38.02%) Frame 1
cellulase; endo-1,4-beta-D-glucanase [Brassica napus]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0065J03
Clone
Clone name
hba-65j3
LE_HBa0065J03
C01HBa0065J03
P065J03
Clone type
BAC
Contamination
none
Estimated length
122,170
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found