Clone Read LE_HBa0227C18_SP6_403269

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0227C18_SP6_403269
Primer
SP6
ID from Seq. Facility
LE_HBa_227_C18-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
2 trimmed for vector and quality, 2470 raw
Sequence
>LE_HBa0227C18_SP6_403269 (raw untrimmed)
AACATCGGATCAGATTGTGGAGCGAACGCGAGAGACTTGACCGACTGACCTGTGCTTGGACTACACATAATACACGAACNTGGCTGCGATACTATTCATG
ACTTGCTCGCTATACCTGTTAGATACAATAGCTCCCGCATACCTGTAGTTGCGCGCAGAGTTACTACAAGATGAACGCGCTACACCGCTAAGCAATCACT
CGATTTCTATCGGCCACTGAGACGGTCTCACTGAACACATTATCCTGCTCTGATTCATTAACTCATGCGACTATCATGCATGTTGACCACGATCTCATCT
TCCGCTGACTGGGCGACACTAAGATACTCATGCGGTATCTCTCTAGGACTCCGTCTCAGATACAGATTCATTGGAGCCCTTCAACTCATAGGAGAATAGA
CAATGATTAACAGACCGCGACCTACGCCCACCCACTAGCTTACGATAAGACACAGGCGACTTCAATACAAGTCCAGTANATCTTGGCCATGACAAGCATA
TGACCTACTAACTCTAACGCATAGCAGATTCGTACTCGTCCACCCTAGTCTCTCACGACCGTGATCACAGCCCTCTCGTTGGCATACTTCATCATTCATC
GTCCCGTCACCTCTATGATGAATATGACACGCTGGCGAAATGGAACTCAACGACGATGATACTTATGGCCTTGCTGAATCGCCAAACTCTCTACACGAGC
AATTCCATGATTTTGAGAGACCGTCTCACCGTAATCTGTTTCACGCGGGTCACGAGAGCACATCTGATGACTCGCTCGGACCCACCCTTCGCCCTACATG
AAACCGGTGGAACATCCACAGACGGCACTGACGACAGCACATCCCGCGCATTCTTAGGCAATGTATTGGCGCTGGAACGTCGAGCATATGCAAGAACTCG
AATACACGCTGAAACGATCATTCGACACGTATGGGAGGCGACACGATAACATTCGACTCTTTGAAACCATCAACCATTCATATGCAGGAACTATACTCGA
AGCTCTAGGACGACTTCTACACTAGACTCATCGACTAGCGCATGCTAGTGCTACCGACGATGTACAAAACCTCACGGGAGCTATGCGCGCTGAATGCATC
ATGCCCCTGCCACATTTAGCCACATGGACACTGTCACGGCGTAAACTTACACTACTTATACCCACATGGTCGTAGACAATCTTAGGGAGTTACGAGTCGC
TCATGTGTATCTCTGCCACTCACACATCAACTCGCTCTGCGATGCTATGACAGTGCTTCAGATCGCATAATCATTATATTGACACTGACGTGCACCAGCG
TGTCTCTCTATGACATTCACATTCTATACTCTGACACTATATCATCATAATCGCTCACGCTTATGTATATACCGCGCAGCGTCTGCCATCCGTCTAGTCT
ATATCGGCTCACCGTAATAACATAGAGCGAGACATGTCTACTCGCCTGTCTTATTACTCANCTCTGCTCGACACTCGCACATCGCAGCGCTGTATGAGCC
GACACTNCGTACCAATATGCATTCTTTCTATGACTGACCTCAGTCGTGCAATACTGANGACACGCTCTCTCCNACACTGACTATGCACACGCGCATCAAC
GTGCGGTNATGCCGCGCGCGAGTACAACGCCAACCTTGTATGACTCCATAGCNCTGAACATCCGCAATCTGAAAGCTATCTCTGTATTTACGTGCGTGAA
CACACATATGACAACGGGGACTGCATAGTGGCAGTCCGGAACAACAAATGTGGCGCGTACGAACAGTCACTACTATGCCTCTGACTAACGCTTGACCGAT
GACGTGATGATTCTCATACACATAGCTGTGTTCACACTCTTACTCCCTACTCCATCTCAAATCCAGAGTCTCNATACGTATACCTAANTGTCTTGCACTC
TACAGTCTCTGTGATGATTCCACCTGCGCGCTACTACATACGAACAGACATCATGTNGAAGATGCGGCGGCATGAATACACAGATCTGCAACCCTGTTAG
CATCATCTCGCTGAACTGATTACCATACTACTACCAGACTGCTCTGTGTGCTCATCACACGTCGGTCACATTATTGGTACGATCTACACTCCAACCGCCT
ATNCGCTGGCTTATATGCTACTGACTACTGTGGATGAGTCGCCTCTAATCTATGATTCATAATCTACACAGTTCTCACGAAGAACACATGAGCCTGCATT
GTCGCATCTAGTCTACTCATCACCGCACAGNAGTGCATCATCTGATGCAAATGTTGCCTCACACTACTACGTCTGTACTGCGCTCTGCACGCAAGCATAT
TCTTACTCTACACACGCTGCGTGATGCACTCGTCTATCGATTCGTTCCACCAGATGATGACAGTCTCCGTGCTCGCGCTTGATCTCTATACACATTTGCT
GCTACCTCCACGTGACTGTATGCCCAGCACTCATGAGGCGACACGTGTGGCATGACAACACAATGTCACG
>LE_HBa0227C18_SP6_403269 (trimmed for quality and cloning vector)
TC

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
 Warnings:
  • Not screened for repetitive elements
  • Not screened for contaminants
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
2.3877
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0227C18
Clone
Clone name
hba-227c18
LE_HBa0227C18
P227C18
Clone type
BAC
Contamination
none
Estimated length
108,178
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found