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Clone Read LE_HBa0117L07_T7_40093

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0117L07_T7_40093
Primer
T7
ID from Seq. Facility
LE_HBa_117_L07-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
773 trimmed for vector and quality, 915 raw
Genbank Accession
DU060361
Sequence
>LE_HBa0117L07_T7_40093 (raw untrimmed)
ATTAGCTTGGGTCGAGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTTGCTCATTGGTAATGGTTCCGGTTAAGAAGCAGAAAAGGAGGCCG
CCTACACCGTTGGATGCATAGGAAGTAGCATTTAGAAGTAGTGTGACGCAGCGTTTGTAGATCTGATGACCTATGTGGGTGTCTTATCAAGCATTCTTGG
CGAGTTGCAAAAATATTTAGTTGGAACAGGATCAGTCTTTTCCTTTTAATCGGAGAAGATCACTAGTCCTTTGTCGAAAAATTGTACTGTCTGACCGCAT
TTGATTGATCATAAGGGAAAAATTAATGAAATGAAGTGCTACTATAGCAAGAGCTTGGGGTACTTGATATTAAAATTGATGAAGTAGCAACTGAAATCTG
TATTGTATTAGTTATACAGTTCCTTGAAATTTGCACTGTATCAATGTCAGTAATTCAATTTGTGTTATGAGATACACTTGTAAGAACTATCACCCTAAGT
TCATGTCTTGATTTTCTAGATACAACTCAAGAACAACCTAGCTAACTTTGAAAGATTAACCCCATAAACATTTCCAAGTTGAAGGGATTATGGAGATTAG
GTTAGTGTAGTGGATGCTCTTAGCTCTTACCTTAGAACCTCTCTGGGATAATGTGTATTTCTTCAAATGTTGATTGTTGATTGGTAATCTATCCAATGTT
TGATCAATCACATAATATAGTTGATTAAATCCCTTCTTTCTTCCATCTCATCTATTTATTGTGTGTTTAATTGACCTCAAAAATGGGCTGTTTTGGCCGT
TAGGGTCAACCGGCTCCACGGCTAAGGTATAACATACCACTAGAAAAATATTGACATTTAAAACAGGGGGTATGCCTGCTTCAGGGCTGTTTGACCTCGA
AATGGGCATGTTTGC
>LE_HBa0117L07_T7_40093 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTTGCTCATTGGTAATGGTTCCGGTTAAGAAGCAGAAAAGGAGGCCGCCTACACCGTTGGATGCATAGG
AAGTAGCATTTAGAAGTAGTGTGACGCAGCGTTTGTAGATCTGATGACCTATGTGGGTGTCTTATCAAGCATTCTTGGCGAGTTGCAAAAATATTTAGTT
GGAACAGGATCAGTCTTTTCCTTTTAATCGGAGAAGATCACTAGTCCTTTGTCGAAAAATTGTACTGTCTGACCGCATTTGATTGATCATAAGGGAAAAA
TTAATGAAATGAAGTGCTACTATAGCAAGAGCTTGGGGTACTTGATATTAAAATTGATGAAGTAGCAACTGAAATCTGTATTGTATTAGTTATACAGTTC
CTTGAAATTTGCACTGTATCAATGTCAGTAATTCAATTTGTGTTATGAGATACACTTGTAAGAACTATCACCCTAAGTTCATGTCTTGATTTTCTAGATA
CAACTCAAGAACAACCTAGCTAACTTTGAAAGATTAACCCCATAAACATTTCCAAGTTGAAGGGATTATGGAGATTAGGTTAGTGTAGTGGATGCTCTTA
GCTCTTACCTTAGAACCTCTCTGGGATAATGTGTATTTCTTCAAATGTTGATTGTTGATTGGTAATCTATCCAATGTTTGATCAATCACATAATATAGTT
GATTAAATCCCTTCTTTCTTCCATCTCATCTATTTATTGTGTGTTTAATTGACCTCAAAAATGGGCTGTTTTG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.962806
Expected error %
0.391579
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65305.1:1-137 Score 66 E Value 6e-12 Identity 100.00% Align. Length 32bp (4.14%) Frame 2
Cloning vector pGEM-4Z
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65302.1:1-99 Score 66 E Value 6e-12 Identity 100.00% Align. Length 32bp (4.14%) Frame 2
Cloning vector pGEM-3
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0117L07
Clone
Clone name
hba-117l7
LE_HBa0117L07
P117L07
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found