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Clone Read LE_HBa0222C11_T7_398609

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0222C11_T7_398609
Primer
T7
ID from Seq. Facility
LE_HBa_222_C11-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
561 trimmed for vector and quality, 907 raw
Sequence
>LE_HBa0222C11_T7_398609 (raw untrimmed)
CCTTTAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGCCAAATTGTTATCCGCTCACAATTCCACACAACATACGAGTCCGGAAGCATAAAGTGTA
AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG
AATCGGCCAACGCGAACCCCTTGCGGCCGCCCGGGCCGTCGACCAATTCTCATGTTTGACAGCTTATCATCGAATTTCTGCCATTCATCCGCTTATTATC
ACTTATTCAGGCGTAGCAACCAGGCGTTTAAGGGCACCAATAACTGCCTTAAAAAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCA
TTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCA
TGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAGAACATATTCTC
AATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCCGAAATCGTCGGGGTATTCACTC
CAGAGCGAAGAAAACGTTTCAGTTTGCTTCAGGAAAACGGTGTAACAAGGGTGGAACACTATCCCATATCACCAGCTCACCGGCTTTCATTGGCCTTACG
GAATTCCGGATGAACATTCATCCGGGGGGGCAAAAATGTAAATAAGGCCCGGAAAAAACTTGGGCCTTATTTTTCTTTAAGGGCCTTTAAAAGGCCGTAA
TTTTCCA
>LE_HBa0222C11_T7_398609 (trimmed for quality and cloning vector)
GGCGTAATCATGGTCATAGCTGTTTCCTGTGCCAAATTGTTATCCGCTCACAATTCCACACAACATACGAGTCCGGAAGCATAAAGTGTAAAGCCTGGGG
TGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAA
CGCGAACCCCTTGCGGCCGCCCGGGCCGTCGACCAATTCTCATGTTTGACAGCTTATCATCGAATTTCTGCCATTCATCCGCTTATTATCACTTATTCAG
GCGTAGCAACCAGGCGTTTAAGGGCACCAATAACTGCCTTAAAAAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTC
TGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAAC
GGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.97717
Expected error %
0.352996
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|NGB00026.2:1029-2499-49 Score 458 E Value 1e-130 Identity 97.20% Align. Length 251bp (44.74%) Frame 2
New England BioLabs cloning vector pGPS2
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|J01636.1:1-7477 Score 369 E Value 1e-103 Identity 98.50% Align. Length 201bp (35.83%) Frame 1
E.coli lactose operon with lacI, lacZ, lacY and lacA genes
E. coli K12 Genome (E.coli_K12/Ecoli_genome)NCBI BLAST
gi|16127994|ref|NC_000913.1| Score 369 E Value 1e-102 Identity 98.50% Align. Length 201bp (35.83%) Frame 1
Escherichia coli K12, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0222C11
Clone
Clone name
hba-222c11
LE_HBa0222C11
P222C11
Clone type
BAC
Contamination
none
Estimated length
139,881
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found