Clone Read LE_HBa0234I17_SP6_392426

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0234I17_SP6_392426
Primer
SP6
ID from Seq. Facility
LE_HBa_234_I17-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
587 trimmed for vector and quality, 1030 raw
Sequence
>LE_HBa0234I17_SP6_392426 (raw untrimmed)
CCCCCCCACGCGCAANNGNNNNNNNNGNNANGGNNAATGACTCTATGAGATTGAATCTCCTATTGTTCCTCAGTAGCTCAATGGTAGATTGGTTGGTTGT
TAATCGATTGGTCATTGGTTCGAATCCTACTTGGGGAGATCTAATTGATTCAAAATTCTTTAATCAGAATAAGAATAAAGGGGCTCGCTTTGCCCGTTAA
GAGTAGGTAACCGTTCCATGTCTTTGTTTCTATTGCATTCTATCTCATCGTATCACATTCTGTTCTACGAGATTAGAAAATCACCATCAATACCTCGGTC
TAGGTCCGAGATAATCCTTTGTTCCATAGCCCTGGGGCTATTTTCAACTAGACAATTAAGAAGTCTCAGATGTACTAGCACTGCATATTTGATGCAATCA
TCGATTCTCCCTAGAGGTCACAATTGCCGTGAGCAAAGATAGTAAAGACGAGGAAGGCTTTTTTGTTATGCTACTAATACTTGCTCTGCTATTCTGCCCA
AGCCTGGCTGAGGAAGAGTTATAGGGGGTAAAACAAAAAAATACGCCGATCGGGCATACTATGTGTAATAATTCCCCCATTGACGATAAATAAAAAGATA
AAAAGACATTCCATTTCGACAAAAGACCCACACCCAAGTTCCCTAGCTTTTGTTTCCCTATCCCGATCATGTTTTCCTACCCCCCAGAGGGAAAGATGCT
TCCCCTTTTTAGCCGGCTGTGGGATAGGAGGGAATCTAAACCCCTGACACTGTGGTTCGTATCCATGTGCTCCTATCCCTCTGACCACAGGTCCCTCCCC
GTCTCCCGGGGATCTCTTCCTGGGGATCACCCTAAAAAAAGGACCTTTTCCTTTCCCTGAACCTTTCCTGGTTAAGAAAAATGGGAAACCGGGTTCTTCC
TTTTAAATCTCAAAAAAAAGGGGTCCGTTTTCTAGGTTGGGATTGGGAAAAAAAACTGTATATCCCAATTGGGGGGTTCTAAAATAAACCAACCCTTTTT
CCTTTCACTTTGTTTTCGATTTTCAAAAAG
>LE_HBa0234I17_SP6_392426 (trimmed for quality and cloning vector)
TGACTCTATGAGATTGAATCTCCTATTGTTCCTCAGTAGCTCAATGGTAGATTGGTTGGTTGTTAATCGATTGGTCATTGGTTCGAATCCTACTTGGGGA
GATCTAATTGATTCAAAATTCTTTAATCAGAATAAGAATAAAGGGGCTCGCTTTGCCCGTTAAGAGTAGGTAACCGTTCCATGTCTTTGTTTCTATTGCA
TTCTATCTCATCGTATCACATTCTGTTCTACGAGATTAGAAAATCACCATCAATACCTCGGTCTAGGTCCGAGATAATCCTTTGTTCCATAGCCCTGGGG
CTATTTTCAACTAGACAATTAAGAAGTCTCAGATGTACTAGCACTGCATATTTGATGCAATCATCGATTCTCCCTAGAGGTCACAATTGCCGTGAGCAAA
GATAGTAAAGACGAGGAAGGCTTTTTTGTTATGCTACTAATACTTGCTCTGCTATTCTGCCCAAGCCTGGCTGAGGAAGAGTTATAGGGGGTAAAACAAA
AAAATACGCCGATCGGGCATACTATGTGTAATAATTCCCCCATTGACGATAAATAAAAAGATAAAAAGACATTCCATTTCGACAAAA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.974114
Expected error %
0.248727
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 630 E Value 0 Identity 92.60% Align. Length 584bp (99.49%) Frame 1
Nicotiana tabacum plastid, complete genome
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 82 E Value 5e-17 Identity 85.10% Align. Length 100bp (17.04%) Frame 1
No sequence description found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0234I17
Clone
Clone name
hba-234i17
LE_HBa0234I17
P234I17
Clone type
BAC
Contamination
none
Estimated length
111,380
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found