Clone Read LE_HBa0111M10_SP6_36306

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0111M10_SP6_36306
Primer
SP6
ID from Seq. Facility
LE_HBa_111_M10-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
131 trimmed for vector and quality, 846 raw
Sequence
>LE_HBa0111M10_SP6_36306 (raw untrimmed)
ACGGTCTTATGCTCAATCCTACAGAACCAAATTCCATTATTAAGGATCACGGATAATGACCAGGATCATTGTGATGCCCATAAAGTGCAAAATTTGCTCT
GAGACGTTTCCAGATGAGCAAGTGCTTGGCACACACTGGATGGACAGCCACAAAAAGGAAGCCCAATGGCTTTTCAGGGGTTATGCTTGTGCTATCTGTC
TGGATTCTTTTACTAATAAGAAAGTTTCGGAAACTCATGTGCAGGAAAGGCACCATTCACATTTCGTTGAAAATTGCATGCTTTTTCATTGTATTCCGTG
TATTAGCTATTTTGGGAACTCATTAGGAGTTATGGCCCCACGCGCTCCCCTCACATCCTTCCAGTTTGATCTGGTCTCCCACAGCTCAAGAGAATTATTT
TCCCGCAGATGAGGTTGCATCAGAGAAACCTGATATATTGCAACTCCTTGTCGACACCCAACTTCAACTCTCACAATCAGTTCTGGCTTCCTAACATTCT
TCTCCACATATTGCGGCCTGATATCTGACTTGTCTACTTTTTCTTTGCCGTCATTCCTTCCGCTGTTCTTTCTATGTTTCCTAACCTGATGTCTACCCTT
ATCCCCAACATGTCTATTCAATTTCAATATACGCTATAAATCCCCCAACCGTACCACTGACCTCCCCTCTACATTCAACATATGCTCCCCCACCCTCATT
TCCTATTCTTTCTTTTCTATTAACCCCCCTCCCTCATTCTCTAACTTTCATCCTTTTCCTGCCTTCCTAACTGCTATTTCCTTTCCTCCTACTTCTCCCT
CCACCCTTCTCTCATCTTCTCTAATCTCTCCCTTTCTCCTTCTTTT
>LE_HBa0111M10_SP6_36306 (trimmed for quality and cloning vector)
TTTCCAGATGAGCAAGTGCTTGGCACACACTGGATGGACAGCCACAAAAAGGAAGCCCAATGGCTTTTCAGGGGTTATGCTTGTGCTATCTGTCTGGATT
CTTTTACTAATAAGAAAGTTTCGGAAACTCA

This is probably not a useful sequence.

Problems:
  • Short cloning insert
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.921783
Expected error %
0.504511
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAO32935.1| Score 71 E Value 8e-12 Identity 23.00% Align. Length 125bp (95.42%) Frame 2
SET domain protein SDG117 [Zea mays]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_467404.1| Score 70 E Value 1e-11 Identity 21.40% Align. Length 125bp (95.42%) Frame 2
putative SET domain protein SDG117 [Oryza sativa (japonica cultivar-group)] dbj|BAD08114.1| putative SET domain protein SDG117 [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0111M10
Clone
Clone name
hba-111m10
LE_HBa0111M10
C02HBa0111M10
P111M10
Clone type
BAC
Contamination
none
Estimated length
138,131
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found