Clone Read LE_HBa0196N05_T7_259734

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0196N05_T7_259734
Primer
T7
ID from Seq. Facility
LE_HBa_196_N05-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
554 trimmed for vector and quality, 1015 raw
Genbank Accession
DU083845
Sequence
>LE_HBa0196N05_T7_259734 (raw untrimmed)
GTCGGGGTCTCTGAGTCGACCTGCAGGCATGCAAGCTTGGTCTTAATGATAGAATGTTGCTGGAAGCACAAGGGCGAACTACCAAGGGGAAGGCCATTGA
TCTTGATGATATCAAGTTTCATCAGTATGAAATTTGTTCAGTTATTTCTTTTTGTGGGATTATGATATATTGGTTTCCTGCCAAACGGAGTTATGTGGGT
TGGCCATTGATTAAGTTGCATAACATGCTTCTCAAAGATGAAATACTTTACTAAATAGCGCGGCCTGGAAAGTCAAACCTATCACTTTAAAAGGAATTTA
GTGAAGTATGTGGTTGTTGAACCAGCAACCTGTACCTCCCGCACAATTTTTCAAATGATAAATCCTGGAGGCTTTTATCCATGTCTTGAGTCTTGTCTAG
TTTTGAATAGTAACCCATTTCACTATCTTATTGCCCTTCTCTTTCTCTTCCACTAATTTAGGTGTGTTCGCTTGGCCCGATTTGAAAATGATCGCACTAT
ATCATTTATACCGCCAGATGGAGCCTTTGATCTTATGACCTACAGACTTGGTACTCAGGTAAATTATGAGCTTGTTGGGTCTTGAATTACTTTTAGTTCC
CTTGTGGATGTTCTTAAGCCAAAATTGTTTTGACCCCTGATGGTAATTGTCTCAGGGTAAAGCCTCTCCTATGGGTGGGAAGCTCAAGTGGGAAAGGCAT
TCCTAGGACCCGAATGGAATTTGTTGTTTAAAGCCCGGAAACCCGTTTTCAAAGGAAACAAAGGTTTGAATTTGTTTTTAAGTTATTTACAGTGGAAAAA
CCTCGGCCTAATTTAAAAGGCTCCCATTTTTCCTCTTATACTCGCGGAAATGGCCTATGCTTATATCCTTATAAAAGAAATTTTTCCCAGTGCCCCCGTA
ATGGAGTTTCTTTCAAAAAAATTTCGGGTCTGGCCACTCTAAGGTTTCAAAATTTTAATTTCCCCTGGTGCCAACGCGAGTTCTAACCAATCCTCTATTG
TTACAAAAAACTCGG
>LE_HBa0196N05_T7_259734 (trimmed for quality and cloning vector)
GTCGACCTGCAGGCATGCAAGCTTGGTCTTAATGATAGAATGTTGCTGGAAGCACAAGGGCGAACTACCAAGGGGAAGGCCATTGATCTTGATGATATCA
AGTTTCATCAGTATGAAATTTGTTCAGTTATTTCTTTTTGTGGGATTATGATATATTGGTTTCCTGCCAAACGGAGTTATGTGGGTTGGCCATTGATTAA
GTTGCATAACATGCTTCTCAAAGATGAAATACTTTACTAAATAGCGCGGCCTGGAAAGTCAAACCTATCACTTTAAAAGGAATTTAGTGAAGTATGTGGT
TGTTGAACCAGCAACCTGTACCTCCCGCACAATTTTTCAAATGATAAATCCTGGAGGCTTTTATCCATGTCTTGAGTCTTGTCTAGTTTTGAATAGTAAC
CCATTTCACTATCTTATTGCCCTTCTCTTTCTCTTCCACTAATTTAGGTGTGTTCGCTTGGCCCGATTTGAAAATGATCGCACTATATCATTTATACCGC
CAGATGGAGCCTTTGATCTTATGACCTACAGACTTGGTACTCAGGTAAATTATG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.967308
Expected error %
0.177821
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g60780.1 Score 70 E Value 7e-13 Identity 89.50% Align. Length 531bp (95.85%) Frame 2
68414.m06842 clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit family
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g10730.1 Score 69 E Value 2e-12 Identity 28.90% Align. Length 113bp (20.40%) Frame 3
68414.m01223 clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM20503.1| Score 70 E Value 3e-11 Identity 29.80% Align. Length 113bp (20.40%) Frame 3
clathrin adaptor medium chain protein MU1B, putative [Arabidopsis thaliana] ref|NP_176277.1| clathrin adaptor complexes medium subunit family protein [Arabidopsis thaliana] gb|AAB71967.1| putative Clathrin Coat Assembly protein [Arabidopsis thaliana] gb|AAN72155.1| clathrin adaptor medium chain protein MU1B, putative [Arabidopsis thaliana] pir||C96633 probable Serine/Threonine protein kinase F8A5.29 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF17661.1| Score 69 E Value 7e-11 Identity 28.90% Align. Length 113bp (20.40%) Frame 3
F20B24.16 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0196N05
Clone
Clone name
hba-196n5
LE_HBa0196N05
P196N05
Clone type
BAC
Contamination
none
Estimated length
107,103
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found