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Clone Read LE_HBa0177L08_T7_256528

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0177L08_T7_256528
Primer
T7
ID from Seq. Facility
LE_HBa_177_L08-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
524 trimmed for vector and quality, 980 raw
Sequence
>LE_HBa0177L08_T7_256528 (raw untrimmed)
TGGTCGGGGATCTCTGAGTCGACCTGCAGGCATGCAAGCTTCCGGTTAGCTAAACTGATGAAATTCTAGATTTCAAACTTTCTTGTACCTTGGGTAAGGA
TTCTATATAATTTTCAGGAACCTCCATCTCTGTAAGAACTTGAGCATTTATGGCGAGGGCTGCTTTATGAACTTGGTTCACCGAATCTTTCTGAAATTGC
AGCCATTTCCTAGTAGTATCGGACAAACCATTAGGAGGAACCTTAGGGGTAGGTATCCACCATTTATCATCTTTCCTGGCAGTTTTACCTTCATCATCTT
TGGATGTATAACAGATTTCTGTTTGGTCTTTAAAACTATCTAAACAATCCTGTTTGTTGTAAAACCAAACATAAGCATAATTAAGAGTAAACTGCATTAT
TTAAAATGACATAAGCTAACCAAAAAAAAAAACACAGAGAAATGAAAAGCAATGCAAAGAAAGAAAAGAAGTTTTCTCCCTACCAGAAGCATTGTATCTA
GCTTACGCAATGCGGGAATGTTCATTTGAAGATCAGTTCTCTGCTTTGTCACCATAATCTGAAAAAATTAAGCATTTGATGCTTTTGTTAGTTTGTTTTA
TATTAATATAAATGACAGATGTGAAGAGGAATTCCCAGTTTGAGACCGATGTTTGTATGCCTCCCAAATTGTTCCCCCTTTAGATTTGTGTTTGCATCTA
CCCATTTCCCCTTACTATCCTTTACCCATATAAGCCATTTATTTTCTTTTTTCTCCTTGTTCTCTCTCTCTGGGTGCTCGGGTTGCAATCTCCTCCCGTT
TCCCAAATGCTTGAACCCTGGTTTCTTGATACCTGTATCCAACTGTTTGATCCCAACCACTGTATTTTCCAACTGTTTCCGGACTAATTACGTTCTATCT
CTTACATGCTGGTTACTGCCACTTTTGCTCTACTCCCCTTCGTTTCCCTCCTTTCACCCCCCCTATCATCCTTATCAGTG
>LE_HBa0177L08_T7_256528 (trimmed for quality and cloning vector)
CTGCAGGCATGCAAGCTTCCGGTTAGCTAAACTGATGAAATTCTAGATTTCAAACTTTCTTGTACCTTGGGTAAGGATTCTATATAATTTTCAGGAACCT
CCATCTCTGTAAGAACTTGAGCATTTATGGCGAGGGCTGCTTTATGAACTTGGTTCACCGAATCTTTCTGAAATTGCAGCCATTTCCTAGTAGTATCGGA
CAAACCATTAGGAGGAACCTTAGGGGTAGGTATCCACCATTTATCATCTTTCCTGGCAGTTTTACCTTCATCATCTTTGGATGTATAACAGATTTCTGTT
TGGTCTTTAAAACTATCTAAACAATCCTGTTTGTTGTAAAACCAAACATAAGCATAATTAAGAGTAAACTGCATTATTTAAAATGACATAAGCTAACCAA
AAAAAAAAACACAGAGAAATGAAAAGCAATGCAAAGAAAGAAAAGAAGTTTTCTCCCTACCAGAAGCATTGTATCTAGCTTACGCAATGCGGGAATGTTC
ATTTGAAGATCAGTTCTCTGCTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.959899
Expected error %
0.212348
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g79860.1 Score 124 E Value 4e-29 Identity 23.00% Align. Length 260bp (49.62%) Frame 1
68414.m09329 expressed protein contains Pfam profile PF03759: Domain of unknown function (DUF315)
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g52240.1 Score 119 E Value 7e-28 Identity 21.50% Align. Length 260bp (49.62%) Frame 1
68414.m05894 expressed protein contains Pfam profile PF03759: Domain of unknown function (DUF315)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAW57535.1| Score 186 E Value 3e-46 Identity 33.30% Align. Length 260bp (49.62%) Frame 1
pollen-specific kinase partner protein [Lycopersicon esculentum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_178104.1| Score 124 E Value 2e-27 Identity 23.00% Align. Length 260bp (49.62%) Frame 1
expressed protein [Arabidopsis thaliana] gb|AAG52233.1| hypothetical protein; 60088-62008 [Arabidopsis thaliana] pir||F96829 hypothetical protein F19K16.18 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0177L08
Clone
Clone name
hba-177l8
LE_HBa0177L08
C03HBa0177L08
P177L08
Clone type
BAC
Contamination
none
Estimated length
88,308
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found