Clone Read LE_HBa0161E16_SP6_201427

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0161E16_SP6_201427
Primer
SP6
ID from Seq. Facility
LE_HBa_161_E16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
149 trimmed for vector and quality, 788 raw
Sequence
>LE_HBa0161E16_SP6_201427 (raw untrimmed)
CAAAACCACATCCCTATATTGTGTAAATCTTTTGATAAGAGGTACTACTTAGGGAAATTTTTCTACGATAATTTTGCTTCTAAATTACTTTTCAACAGGA
TAGCTATACATAATAAACGCCTTTTTTTGCTACAGTCTCGAGCGCATCCACGTGAAATAATGACTGAAGTTTTGAAAGCTCTGCAAGAACTGAATGTATG
TTGGAAAAAGATTGGTCAGTATAACATGAAATGTCGATGGGTTCCTAGCTTACCTGGTCATCATGAAGGCATGGGTGTTAATTCCATGCATGGGAATTAG
TTCTTTGGAGTTGATTCTTCCTTCATTGATAATGATGGGGCCACATAGTTAACTTATGCGATCTAGTGTAGAGTTCAGGTTCAATATTTTCATGCATCCA
ATATCTATTTACTTTGAGATTTCCTTCTATTCTGTTACTTGATTTTCTCTTTTCCCTGTCCTAGGACCTACATAGTCTTGTTAATGTAGAGTTTTATTAT
TTTGTTTTTTCTCGTCATTACTCTTCATATTACGTATAACCTCATGAAGTTATCTATTCTTAGTTACTTATTGAATATAATATATCATTGTTTCTAATGA
TTACAAAACGTAAAAATACTGCATGAACATTATGTTCACATTGGAGTGCATCATATTATATGTTATTTATGACTATTTATGTCTCGCACCGTGACCATCG
CATGTCATCAGTAACTGAAACATCAAAGTCTGCCATNAATATAACATTAATAATGCTAATAATGAACTATACATCGTTCCTTATTTTG
>LE_HBa0161E16_SP6_201427 (trimmed for quality and cloning vector)
CTATACATAATAAACGCCTTTTTTTGCTACAGTCTCGAGCGCATCCACGTGAAATAATGACTGAAGTTTTGAAAGCTCTGCAAGAACTGAATGTATGTTG
GAAAAAGATTGGTCAGTATAACATGAAATGTCGATGGGTTCCTAGCTTA

This is probably not a useful sequence.

Problems:
  • Short cloning insert
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.946926
Expected error %
0.392637
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g01090.2 Score 85 E Value 9e-18 Identity 30.80% Align. Length 119bp (79.87%) Frame 1
68416.m00014 Snf1-related protein kinase (KIN10) (SKIN10) identical to Snf1-related protein kinase, KIN10 SP:Q38997 from [Arabidopsis thaliana]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g01090.1 Score 85 E Value 9e-18 Identity 30.80% Align. Length 119bp (79.87%) Frame 1
68416.m00013 Snf1-related protein kinase (KIN10) (SKIN10) identical to Snf1-related protein kinase, KIN10 SP:Q38997 from [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF66639.1| Score 91 E Value 1e-17 Identity 29.30% Align. Length 146bp (97.99%) Frame 1
SNF1 [Lycopersicon esculentum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||T07788 Score 90 E Value 2e-17 Identity 28.60% Align. Length 146bp (97.99%) Frame 1
probable serine/threonine-specific protein kinase (EC 2.7.1.-) SNF1 - potato
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0161E16
Clone
Clone name
hba-161e16
LE_HBa0161E16
C02HBa0161E16
P161E16
Clone type
BAC
Contamination
none
Estimated length
93,916
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found