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Clone Read LE_HBa0028F18_T7_138120

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0028F18_T7_138120
Primer
T7
ID from Seq. Facility
LE_HBa_028_F18-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
851 trimmed for vector and quality, 1006 raw
Genbank Accession
DU047655
Sequence
>LE_HBa0028F18_T7_138120 (raw untrimmed)
TAACGGGGTCCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGAAACAAAAAATTAATATGTTGTTCTCCTCTTGAAAGATATTTGGGAA
TTTCTAAGGGGTTCAATAAACTAATGTGTGAATATTTGATTGTTTCAAAGGTTTGCTGCTATTTGCATCTTTCTAAAATCATTTTTTTGATATGCAGCAG
TCTACCCCTGGAATAGTAAGCATGTCAAGATTAAGGTGTATGCTTCGAGGATTGGATTTAAAGGCTATTGTCTTCTTGGTTGTTTTTGTGCCATTATGTA
TAGTTGGTATATACTTGCATGGACAGAAGATTACCTACTTCCTCCGACCAATATGGCAATCTCCTCCAAAGCCCTTTATTGAACTTACCCATTATTATCA
TGAGAATGTCTCCATGGAGAATCTTTGCAAGCAACATGGATGGGGAATTCGTGAATATCCTAGGCGTGTCTATGATGCTGTTTTGTTTAGTAATGAAGTG
GACATGCTTACTATCAGATGGAAGGAATTGTATCCTTACATCACACAGTTTGTTCTGCTCGAGTCAAACTCCACGTTTACTGGATTACCAAAACCATTTA
CTTTTGCTATTAACCGGGACCAATTTAAGTTTGTTGAGCCTCGATTGACTTATGGAACCATTGGAGGAAGATTCAGGAAAGGTGAAAATCCGTTTGTTGA
AGAGGCATATCAGAGAGTAGCACTCGACCAGCTTTTGAGAATAGCTGGAATAGAGGATGATGATTTGCTGATAATGTCCGATGTTGATGAAATTCCTAAG
CAGGCACACCATCGATCTTTTAAGATGGTGTGATGACATTCCTCCCATTCTTCACCTTTCTTTTAAGGATTACTTGTACTCTTTTGAATTTGAGCTTAGG
CCCAAAGTTGGGAAACTTCAGTCCACAGGTTCAGAAGGGAAGACTCCCTTACTACACTATCCTCAAACTGATTACCTTTGGGAAATTCCGGTTGGCTTGT
ACCTTT
>LE_HBa0028F18_T7_138120 (trimmed for quality and cloning vector)
AGCTTTGAAACAAAAAATTAATATGTTGTTCTCCTCTTGAAAGATATTTGGGAATTTCTAAGGGGTTCAATAAACTAATGTGTGAATATTTGATTGTTTC
AAAGGTTTGCTGCTATTTGCATCTTTCTAAAATCATTTTTTTGATATGCAGCAGTCTACCCCTGGAATAGTAAGCATGTCAAGATTAAGGTGTATGCTTC
GAGGATTGGATTTAAAGGCTATTGTCTTCTTGGTTGTTTTTGTGCCATTATGTATAGTTGGTATATACTTGCATGGACAGAAGATTACCTACTTCCTCCG
ACCAATATGGCAATCTCCTCCAAAGCCCTTTATTGAACTTACCCATTATTATCATGAGAATGTCTCCATGGAGAATCTTTGCAAGCAACATGGATGGGGA
ATTCGTGAATATCCTAGGCGTGTCTATGATGCTGTTTTGTTTAGTAATGAAGTGGACATGCTTACTATCAGATGGAAGGAATTGTATCCTTACATCACAC
AGTTTGTTCTGCTCGAGTCAAACTCCACGTTTACTGGATTACCAAAACCATTTACTTTTGCTATTAACCGGGACCAATTTAAGTTTGTTGAGCCTCGATT
GACTTATGGAACCATTGGAGGAAGATTCAGGAAAGGTGAAAATCCGTTTGTTGAAGAGGCATATCAGAGAGTAGCACTCGACCAGCTTTTGAGAATAGCT
GGAATAGAGGATGATGATTTGCTGATAATGTCCGATGTTGATGAAATTCCTAAGCAGGCACACCATCGATCTTTTAAGATGGTGTGATGACATTCCTCCC
ATTCTTCACCTTTCTTTTAAGGATTACTTGTACTCTTTTGAATTTGAGCTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.957501
Expected error %
0.7824
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g67880.1 Score 323 E Value 1e-100 Identity 73.90% Align. Length 696bp (81.79%) Frame 3
68414.m07751 glycosyl transferase family 17 protein low similarity to beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.144) from Mus musculus [SP|Q10470], Rattus norvegicus [SP|Q02527], Homo sapiens [SP|Q09327] ; contains Pfam profile PF04724 :Glycosyltransferase family 17
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g12990.1 Score 323 E Value 1e-100 Identity 74.40% Align. Length 672bp (78.97%) Frame 3
68414.m01508 glycosyl transferase family 17 protein low similarity to beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.144) from Mus musculus [SP|Q10470], Rattus norvegicus [SP|Q02527], Homo sapiens [SP|Q09327]; contains Pfam profile PF04724 :Glycosyltransferase family 17
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAI70376.1| Score 332 E Value 1e-101 Identity 75.70% Align. Length 693bp (81.43%) Frame 3
beta 1,4 N-acetylglucosaminyltransferase [Populus alba x Populus tremula]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL85033.1| Score 323 E Value 1e-98 Identity 73.90% Align. Length 696bp (81.79%) Frame 3
unknown protein [Arabidopsis thaliana] ref|NP_176955.1| glycosyl transferase family 17 protein [Arabidopsis thaliana] gb|AAG51993.1| unknown protein; 88937-90309 [Arabidopsis thaliana] pir||E96701 unknown protein, 88937-90309 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0028F18
Clone
Clone name
hba-28f18
LE_HBa0028F18
C02HBa0028F18
P028F18
Clone type
BAC
Contamination
none
Estimated length
85,093
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found