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Clone Read LE_HBa0027H13_T7_137438

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0027H13_T7_137438
Primer
T7
ID from Seq. Facility
LE_HBa_027_H13-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
750 trimmed for vector and quality, 896 raw
Genbank Accession
DU045738
Sequence
>LE_HBa0027H13_T7_137438 (raw untrimmed)
CCTCGTACCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTACCTGGTCTCCAGCCTTCACCTTTAATGGACTTCGTAAAGAAAACAACAA
ATCCCATGAGAACAGATTCTGCTGGTCCCCTAACTTCATGGGCATGATCTTCTGTGGTAACTCTTGCATTGTGGTTTGTCTGATAAGTAAACCATTCATA
TAACGTTATTCACACAATCCATCATAGCCAATAAAATGATGAAATGTTATATAACAATGAACATTTACTGCAGGTGCACAGAGCCAGAGCCTCTCTCACC
ATTGTGGGCTTCTTATGAGCAGTCGGCATCATTAAAATACTTCAATTGCTTATCTAAGTAGTTTTCATTTACCTAATATCTTACCAGGTCAGCAAAAGGA
ACCAGAATAAGATTTTGATTCCGAAGACGAGAAAATGCCCTTGATCTGAGCATTCCAAATGCCCAGAAGAAATCATCCAACGTTATAGGAAAAGGGAAAA
GCTGCTTGTTAGGAAGTATGACTTCTTCTTCCACTTTTCGGAATTCATTTTGCACATAATCTTTCACACTCAATGTTGTGCTTAAAAGTTGAGTCCCTGC
AAATGATAGCATGACAAACGAAATGAATCACAAATACCTGAAATTCTCAAATTCTCACTTAACCAAACAAGGGACATCACATTTCTTATATAGCAACGAG
CAATTTCATCATCTTTAGAGAAAATGCCATCGTGTTCTACACAATTGATCATGGAGTGTAATCAAGACAATCATGTAACAACCCTATGCAAAAGCATTAA
CAGTATCTCTTACTAAGTCAAATAAGGATCACAATTTCATAGGTTATATGAAAAACCTTAACTAGCCCTACCATAATAAATATTTGCTTCCAAAGC
>LE_HBa0027H13_T7_137438 (trimmed for quality and cloning vector)
AGCTTTACCTGGTCTCCAGCCTTCACCTTTAATGGACTTCGTAAAGAAAACAACAAATCCCATGAGAACAGATTCTGCTGGTCCCCTAACTTCATGGGCA
TGATCTTCTGTGGTAACTCTTGCATTGTGGTTTGTCTGATAAGTAAACCATTCATATAACGTTATTCACACAATCCATCATAGCCAATAAAATGATGAAA
TGTTATATAACAATGAACATTTACTGCAGGTGCACAGAGCCAGAGCCTCTCTCACCATTGTGGGCTTCTTATGAGCAGTCGGCATCATTAAAATACTTCA
ATTGCTTATCTAAGTAGTTTTCATTTACCTAATATCTTACCAGGTCAGCAAAAGGAACCAGAATAAGATTTTGATTCCGAAGACGAGAAAATGCCCTTGA
TCTGAGCATTCCAAATGCCCAGAAGAAATCATCCAACGTTATAGGAAAAGGGAAAAGCTGCTTGTTAGGAAGTATGACTTCTTCTTCCACTTTTCGGAAT
TCATTTTGCACATAATCTTTCACACTCAATGTTGTGCTTAAAAGTTGAGTCCCTGCAAATGATAGCATGACAAACGAAATGAATCACAAATACCTGAAAT
TCTCAAATTCTCACTTAACCAAACAAGGGACATCACATTTCTTATATAGCAACGAGCAATTTCATCATCTTTAGAGAAAATGCCATCGTGTTCTACACAA
TTGATCATGGAGTGTAATCAAGACAATCATGTAACAACCCTATGCAAAAG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.957703
Expected error %
0.4085
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g14030.1 Score 114 E Value 8e-26 Identity 23.40% Align. Length 221bp (29.47%) Frame 3
68414.m01658 ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase, putative strong similarity to SP|P94026 Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N- methyltransferase, chloroplast precursor (EC 2.1.1.127) ([Ribulose- bisphosphate-carboxylase]-lysine N-methyltransferase) {Nicotiana tabacum}; contains Pfam profile PF00856: SET domain; Rare GC intron splice site at 49572 is inferred from protein alignment and is not confirmed experimentally
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC49566.1| Score 137 E Value 2e-31 Identity 90.50% Align. Length 547bp (72.93%) Frame 1
ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase gb|AAC49565.1| ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas pir||T03759 [ribulose-bisphosphate-carboxylase]-lysine N-methyltransferase (EC 2.1.1.127) - common tobacco sp|P94026|RBMT_TOBAC Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplast precursor ([Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase) (RuBisCO methyltransferase) (RuBisco LSMT) (rbcMT)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
sp|Q43088|RBCMT_PEA Score 118 E Value 1e-25 Identity 23.90% Align. Length 221bp (29.47%) Frame 3
Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplast precursor ([Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase) (RuBisCO methyltransferase) (RuBisco LSMT) (rbcMT) gb|AAA69903.1| ribulose-1,5 bisphosphate carboxylase large subunit N-methyltransferase
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0027H13
Clone
Clone name
hba-27h13
LE_HBa0027H13
P027H13
Clone type
BAC
Contamination
none
Estimated length
92,632
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found