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Clone Read LE_HBa0027H13_SP6_137437

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0027H13_SP6_137437
Primer
SP6
ID from Seq. Facility
LE_HBa_027_H13-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
721 trimmed for vector and quality, 973 raw
Genbank Accession
DU045737
Sequence
>LE_HBa0027H13_SP6_137437 (raw untrimmed)
CCGGGGCCTTCCGGGGAGGAGGCTTNGGTNNGAGAATATCAGCCGGTTGTTCTTTAGTGGGTACATACTGCACAGTCATGGTGCCGGCCTGAACTTCATC
GTGAACAAAGAGACGATCGGTATCAACATGCTTCATTCTGGTGTGTAGGACGGAATTCTTGGCCACACAGATGGTTGATTTGTTGTCACAATAGAGAGCA
GGAACAGTGTGAGTGGCGCGGAGTTCGCGAAGAATATATGTGACCCACATGGTCTCAGCAGCAAGAAAAGCAAGGGTGCGGTATTCAGCTTCAGTCGAGG
ACCGAGAGACCTTGAGTTGTTTTTTTGTACACCAGGAGATCAGGTTCGCCCCAAAAAAACGAGAAACCCCAATGTAGATTTTCTGTCATTTTTATCATTC
GCCCAATCTGAATCTGAGAAACCCCGAAGTTTCAAGTCCCCGGGTCGAATGAGTAAACCACGACCAAGAGTGCCGAAAATGTACATGAGAATGTGTTTTA
GACAATAGTAATCATGTTCACTTGGTTGATGCATGCGCTGAGCAACTCGGTTGACAGCAAACTGGATGTCAGGACGGGTAATGGCCAGATACTGTAGAGC
CCCAATGAGGCTGCGGAAGTGAGTGATATCGGCAAAGGGGGTGTCGGCTCCATTCGTAGACGAAGAGACTGCCATCGGTGTTGGTTGACTGTTGCATTTT
TCCAGTCCAGATTTCTGCAACACATCTCGAGCATATTTTGACTGATGAAGAAACAAGCCGCTGCCTGTCCGAGAAACCTCCATTCCCATAAATAAATGTA
GCAGGGCAAGGTCCTTCATTTTGAAGGTAGTATGCATAGCTCGAGTGACCTCCTGAAAGGAAGCTGCCGTAAATTCCGTTAAAATGATATCATCTACATA
AACAAGAAGAATGAATGTACCGTGGGCGAATGGCGAAGAAACAGAATCCTGTCGTTACCCAACCCTTAAAGCA
>LE_HBa0027H13_SP6_137437 (trimmed for quality and cloning vector)
GAATATCAGCCGGTTGTTCTTTAGTGGGTACATACTGCACAGTCATGGTGCCGGCCTGAACTTCATCGTGAACAAAGAGACGATCGGTATCAACATGCTT
CATTCTGGTGTGTAGGACGGAATTCTTGGCCACACAGATGGTTGATTTGTTGTCACAATAGAGAGCAGGAACAGTGTGAGTGGCGCGGAGTTCGCGAAGA
ATATATGTGACCCACATGGTCTCAGCAGCAAGAAAAGCAAGGGTGCGGTATTCAGCTTCAGTCGAGGACCGAGAGACCTTGAGTTGTTTTTTTGTACACC
AGGAGATCAGGTTCGCCCCAAAAAAACGAGAAACCCCAATGTAGATTTTCTGTCATTTTTATCATTCGCCCAATCTGAATCTGAGAAACCCCGAAGTTTC
AAGTCCCCGGGTCGAATGAGTAAACCACGACCAAGAGTGCCGAAAATGTACATGAGAATGTGTTTTAGACAATAGTAATCATGTTCACTTGGTTGATGCA
TGCGCTGAGCAACTCGGTTGACAGCAAACTGGATGTCAGGACGGGTAATGGCCAGATACTGTAGAGCCCCAATGAGGCTGCGGAAGTGAGTGATATCGGC
AAAGGGGGTGTCGGCTCCATTCGTAGACGAAGAGACTGCCATCGGTGTTGGTTGACTGTTGCATTTTTCCAGTCCAGATTTCTGCAACACATCTCGAGCA
TATTTTGACTGATGAAGAAAC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.980849
Expected error %
0.1879
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23160.1 Score 90 E Value 1e-37 Identity 42.40% Align. Length 697bp (96.67%) Frame 3
68417.m03342 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD21687.1| Score 137 E Value 2e-54 Identity 54.10% Align. Length 718bp (99.58%) Frame 3
Strong similarity to gi|3600044 T12H20.12 protease homolog from Arabidopsis thaliana BAC gb|AF080119 and is a member of the reverse transcriptase family PF|00078 pir||C86438 hypothetical protein F28K20.17 - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAK51235.1| Score 133 E Value 8e-53 Identity 51.10% Align. Length 718bp (99.58%) Frame 3
polyprotein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0027H13
Clone
Clone name
hba-27h13
LE_HBa0027H13
P027H13
Clone type
BAC
Contamination
none
Estimated length
92,632
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found