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Clone Read LE_HBa0025I09_T7_135942

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0025I09_T7_135942
Primer
T7
ID from Seq. Facility
LE_HBa_025_I09-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
782 trimmed for vector and quality, 993 raw
Genbank Accession
DU045086
Sequence
>LE_HBa0025I09_T7_135942 (raw untrimmed)
GGCAAACTGGGACCCGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCCCCTCCATCAAAGGCTTCTATGTGACAACACTTTTCATAACAACAG
GAAAGTAGCTATATATCCTCACGTCTAAACTCTACTATTTCATTTCACCGGGCAAAATCCAACTGTAATTCTTACCGTATTTTTCAGTTGTGCCCCCGCC
CCAAAACTAGCCTGTCCAGCTGGAATAGGAAGAACATCGGCATCACTAATGGGGTCTGATATAGGATCTGAAGGAATCTCATCAGCAGACTCTCGAAGGA
TTGCATTGAACATAGCTACATCCAGTCTAGCCACACATTGTTCCATTATCTGGTTACAAGAATGACATTCTCAGCAAAAAGACAGAAATATTATAGAATA
TCAAGAGCATGTTAAGTTTTACTCAGACTAATAATATTACATGTAATAAGTAGTTAAAATAGATTTGAAGGACAATGTGCCAGCTAGTACCAGTTTAGAT
AGAAAACGCAAACAACCACACTCGTGCCCTCCAGCTCTAACAGGGCAAATTCTTTCACATGCATCCTTGAAGGCTTTCTTCCAAAGCTCTGAAGAATAAC
TTCCATGCTCTTCATTATCTGAAGTTGCTGTCCTTCTATAAACTTTGCTTATCAAAGAATTCATACTTTCGCGGATTTCTTTTGCAGCACCAGATTGCAT
ATGTGGAGTGAGAGTCTGAAAAAACAAAAGGGCAGTTTGTATTAATGAATTAGATAATATATTCTTCTATTTTCAAAGTTCAAATGGACCCTGAAAGAAA
GACCTGCCACCAAATAGATTCAACGATAAGGGAGAATATCCAGGCCTCTGTTCTCTGAAGTGCTCTCGTAAATGTACGAAGGGCCTCCCAATTACCAAAA
CTTTCAAAATATCATCCCTTTTAACATTGCTTGGAAAAGTCTCCCCTTTAATGGGGAAAAATTCTTTCTTTTGTCCTTGGGAAACGCCTCTCC
>LE_HBa0025I09_T7_135942 (trimmed for quality and cloning vector)
AGCTTCCCCTCCATCAAAGGCTTCTATGTGACAACACTTTTCATAACAACAGGAAAGTAGCTATATATCCTCACGTCTAAACTCTACTATTTCATTTCAC
CGGGCAAAATCCAACTGTAATTCTTACCGTATTTTTCAGTTGTGCCCCCGCCCCAAAACTAGCCTGTCCAGCTGGAATAGGAAGAACATCGGCATCACTA
ATGGGGTCTGATATAGGATCTGAAGGAATCTCATCAGCAGACTCTCGAAGGATTGCATTGAACATAGCTACATCCAGTCTAGCCACACATTGTTCCATTA
TCTGGTTACAAGAATGACATTCTCAGCAAAAAGACAGAAATATTATAGAATATCAAGAGCATGTTAAGTTTTACTCAGACTAATAATATTACATGTAATA
AGTAGTTAAAATAGATTTGAAGGACAATGTGCCAGCTAGTACCAGTTTAGATAGAAAACGCAAACAACCACACTCGTGCCCTCCAGCTCTAACAGGGCAA
ATTCTTTCACATGCATCCTTGAAGGCTTTCTTCCAAAGCTCTGAAGAATAACTTCCATGCTCTTCATTATCTGAAGTTGCTGTCCTTCTATAAACTTTGC
TTATCAAAGAATTCATACTTTCGCGGATTTCTTTTGCAGCACCAGATTGCATATGTGGAGTGAGAGTCTGAAAAAACAAAAGGGCAGTTTGTATTAATGA
ATTAGATAATATATTCTTCTATTTTCAAAGTTCAAATGGACCCTGAAAGAAAGACCTGCCACCAAATAGATTCAACGATAAG

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.964595
Expected error %
0.6897
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g01810.1 Score 127 E Value 3e-30 Identity 41.60% Align. Length 654bp (83.63%) Frame 3
68416.m00123 expressed protein
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g43230.1 Score 115 E Value 4e-26 Identity 40.00% Align. Length 654bp (83.63%) Frame 3
68418.m05283 hypothetical protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF03435.1| Score 127 E Value 1e-28 Identity 41.60% Align. Length 654bp (83.63%) Frame 3
hypothetical protein [Arabidopsis thaliana] dbj|BAC42049.1| unknown protein [Arabidopsis thaliana] ref|NP_186830.1| expressed protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAC53933.1| Score 116 E Value 2e-25 Identity 39.50% Align. Length 654bp (83.63%) Frame 3
hypothetical protein [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0025I09
Clone
Clone name
hba-25i9
LE_HBa0025I09
C02HBa0025I09
P025I09
Clone type
BAC
Contamination
none
Estimated length
94,652
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found