Clone Read LE_HBa0024G18_T7_135096

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0024G18_T7_135096
Primer
T7
ID from Seq. Facility
LE_HBa_024_G18-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
776 trimmed for vector and quality, 974 raw
Genbank Accession
DU047039
Sequence
>LE_HBa0024G18_T7_135096 (raw untrimmed)
CCAAACCCGGTCCCGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGACTGACCATATGCAGACAGTCTATTATACCTGAGGATCATTCATCC
ACATATGTCAAAGACCTTTGACAACGTCTTTTGGCATAAGCACATTTCATCTATTTTCTGGAAACAACACATGTAGACACTCCTCATGACTGCACATAAA
TGATCAATAATTTTGGCTTAAACATCTTTGTCGCTCTCATCACTCAGAATTAGGGAACAATTATTCTCCAAATATTTTAAATTCGTGAAGGGCATAAGTT
TTTTCCAGCAGTCACTGGAAGTAGCACTGGTTATAATGTTTTTTCATCCCAACTTGGTCGATGATAAGACAACCAGAGAAATTAGATCCTGTTTCTCGCA
GATACTCCGTCCTAATGAGAGCAGATTAGGAGATCACAATGATAAGTTCCTTACACTCAGTTCTTTACTGATACTCTCTGCAGTGTGCAGATCTTCTGCC
ATTGTGGGACACTAATATATACACTAGTCAAATTTGTATGGAAGAAAATCAAAGACTCAACAACTAGAGAATTAGCACTCAAAAGATATGTCAGATTATA
GCATGTTCACCCTGATTGATCATTAATCTTGTCAAACCGAATCCCTTCATTGTAAGAAAATTTGTGGCGACGTGAGGAGACATTAACAATCCTCCCTTCC
TTTCTACTCTCACGAGCTGTTTTTTTCATGGTGTCCAGCAATAAATTTGTCAAAAGGAAGTGGCCTGCAAAAATATACTCTGTCAATCATCCTTTAATGT
GATGCAATTTGACTTCAATATCACCAAAAACATAATTTGAACACCAGATGTCAGGAATCATGGAGAAATCTAAGATATACAAGCTAACCAAACAAGGAGA
ACTATGAAAAAAAAAAATTACATTCGCCTAAAAAACCCACTAGGAACCCCATTAAGGGAAACTAAATAGAAAAA
>LE_HBa0024G18_T7_135096 (trimmed for quality and cloning vector)
AGCTTTGACTGACCATATGCAGACAGTCTATTATACCTGAGGATCATTCATCCACATATGTCAAAGACCTTTGACAACGTCTTTTGGCATAAGCACATTT
CATCTATTTTCTGGAAACAACACATGTAGACACTCCTCATGACTGCACATAAATGATCAATAATTTTGGCTTAAACATCTTTGTCGCTCTCATCACTCAG
AATTAGGGAACAATTATTCTCCAAATATTTTAAATTCGTGAAGGGCATAAGTTTTTTCCAGCAGTCACTGGAAGTAGCACTGGTTATAATGTTTTTTCAT
CCCAACTTGGTCGATGATAAGACAACCAGAGAAATTAGATCCTGTTTCTCGCAGATACTCCGTCCTAATGAGAGCAGATTAGGAGATCACAATGATAAGT
TCCTTACACTCAGTTCTTTACTGATACTCTCTGCAGTGTGCAGATCTTCTGCCATTGTGGGACACTAATATATACACTAGTCAAATTTGTATGGAAGAAA
ATCAAAGACTCAACAACTAGAGAATTAGCACTCAAAAGATATGTCAGATTATAGCATGTTCACCCTGATTGATCATTAATCTTGTCAAACCGAATCCCTT
CATTGTAAGAAAATTTGTGGCGACGTGAGGAGACATTAACAATCCTCCCTTCCTTTCTACTCTCACGAGCTGTTTTTTTCATGGTGTCCAGCAATAAATT
TGTCAAAAGGAAGTGGCCTGCAAAAATATACTCTGTCAATCATCCTTTAATGTGATGCAATTTGACTTCAATATCA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.958956
Expected error %
0.2974
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23420.2 Score 86 E Value 2e-17 Identity 25.50% Align. Length 152bp (19.59%) Frame 3
68417.m03376 short-chain dehydrogenase/reductase (SDR) family protein similar to WW-domain oxidoreductase [Mus musculus] GI:6934274, WW domain-containing oxidoreductase isoform FORII [Homo sapiens] GI:15667686; contains Pfam PF00106: oxidoreductase, short chain dehydrogenase/reductase family
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23420.1 Score 86 E Value 2e-17 Identity 25.50% Align. Length 152bp (19.59%) Frame 3
68417.m03375 short-chain dehydrogenase/reductase (SDR) family protein similar to WW-domain oxidoreductase [Mus musculus] GI:6934274, WW domain-containing oxidoreductase isoform FORII [Homo sapiens] GI:15667686; contains Pfam PF00106: oxidoreductase, short chain dehydrogenase/reductase family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAS38575.1| Score 91 E Value 3e-17 Identity 28.10% Align. Length 152bp (19.59%) Frame 3
short-chain dehydrogenase Tic32 [Pisum sativum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAN13078.1| Score 86 E Value 1e-15 Identity 25.50% Align. Length 152bp (19.59%) Frame 3
unknown protein [Arabidopsis thaliana] ref|NP_194073.2| short-chain dehydrogenase/reductase (SDR) family protein [Arabidopsis thaliana] ref|NP_974596.1| short-chain dehydrogenase/reductase (SDR) family protein [Arabidopsis thaliana] dbj|BAD44049.1| unknown protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0024G18
Clone
Clone name
hba-24g18
LE_HBa0024G18
P024G18
Clone type
BAC
Contamination
none
Estimated length
104,445
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found