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Clone Read LE_HBa0019J20_T7_132172

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0019J20_T7_132172
Primer
T7
ID from Seq. Facility
LE_HBa_019_J20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
697 trimmed for vector and quality, 929 raw
Genbank Accession
DU049180
Sequence
>LE_HBa0019J20_T7_132172 (raw untrimmed)
CTTCGACCCGGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGAAAAATAAAGATTCTAGTCTCTGAATTATATGTTGTCATTAGGGGAGTTAA
TACGCGATGTACTTTTTTTTCCTCACAAGGTTTTAACCTACTGGATTTTCCTTGTAAGGTTTTTAATGAGGCATCCATAATGCGTATTATTAGATATGTG
TACTCTTTTTCCATCACTAGATTTTTCTTTCACTGGGTTTTATCTACCAAGGCTCTAACTCTCCGGTTTGCATAACTTTTCTGTCGACTCTGGGCTGTCA
ACAGTCTATGCTGAATCATATGAACTTGCTCCATAACATCTCTAAGCAAGTCTATATCCAAAGAGTCCATCTCCGTAGAATCAAACCAACCGATCGGGGA
TCTACACCGTCTGCCATACAATGCCTCAAATGGGGGCATCTGAATACTGAAGTGATAACTATTGTTGTAGGAAAACTATGCTACAGGAAAATGTTGATCC
CATCTAGCACCAAAATCAGTCACACACGCCCGGAGCATGTCCTCTAACACCTGAATCGTTTGCTCAGACTGTCTATCTGTCTGAGGTTGAAAAACTACAC
TTATATCTAACCGAGTACCCAGACCATATTGTAATGCTTTCCAAAAATTAGAAGTGAATAAATGAACCTCGATCTGAAAGAGGAACCCCATGTAGTCCCA
CAATCTGACTGATATATATCTCGACTAACTTTTCTACCCGTATACTTCACCCCAACAGGGAATGAAGTGGGTAGACTTTGGTCAGCCCTGTCCAACACCA
ACCCAAATGGAAGTCCTTAACCACCCCCTTATGGGTAAGCAAACCCCCCCCCAAAAATTCCAATATTAATTCCTTTTTTTCTTCTTTTCAAATTGGGGAA
AAAAGAACAATTCCTTTTTGAAAAAAAAC
>LE_HBa0019J20_T7_132172 (trimmed for quality and cloning vector)
AGCTTGAAAAATAAAGATTCTAGTCTCTGAATTATATGTTGTCATTAGGGGAGTTAATACGCGATGTACTTTTTTTTCCTCACAAGGTTTTAACCTACTG
GATTTTCCTTGTAAGGTTTTTAATGAGGCATCCATAATGCGTATTATTAGATATGTGTACTCTTTTTCCATCACTAGATTTTTCTTTCACTGGGTTTTAT
CTACCAAGGCTCTAACTCTCCGGTTTGCATAACTTTTCTGTCGACTCTGGGCTGTCAACAGTCTATGCTGAATCATATGAACTTGCTCCATAACATCTCT
AAGCAAGTCTATATCCAAAGAGTCCATCTCCGTAGAATCAAACCAACCGATCGGGGATCTACACCGTCTGCCATACAATGCCTCAAATGGGGGCATCTGA
ATACTGAAGTGATAACTATTGTTGTAGGAAAACTATGCTACAGGAAAATGTTGATCCCATCTAGCACCAAAATCAGTCACACACGCCCGGAGCATGTCCT
CTAACACCTGAATCGTTTGCTCAGACTGTCTATCTGTCTGAGGTTGAAAAACTACACTTATATCTAACCGAGTACCCAGACCATATTGTAATGCTTTCCA
AAAATTAGAAGTGAATAAATGAACCTCGATCTGAAAGAGGAACCCCATGTAGTCCCACAATCTGACTGATATATATCTCGACTAACTTTTCTACCCG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.972423
Expected error %
0.7531
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38745.1| Score 194 E Value 5e-52 Identity 67.90% Align. Length 486bp (69.73%) Frame 2
putative polyprotein, 3'-partial [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT66771.1| Score 188 E Value 5e-50 Identity 65.70% Align. Length 486bp (69.73%) Frame 2
putative polyprotein [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0019J20
Clone
Clone name
hba-19j20
LE_HBa0019J20
P019J20
Clone type
BAC
Contamination
none
Estimated length
58,530
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found