Clone Read LE_HBa0015I17_T7_129814

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0015I17_T7_129814
Primer
T7
ID from Seq. Facility
LE_HBa_015_I17-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
853 trimmed for vector and quality, 975 raw
Genbank Accession
DU051149
Sequence
>LE_HBa0015I17_T7_129814 (raw untrimmed)
TGGCCTGGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGTGGCCAACACAAATGAACTCTTCATTTACCACACAGAGACTGTTGAG
CTAAAATGTGATCACCTCATCTAAACGGGGATAGGCTCAGGAGCCGGATCCGAGGGAGTACCACTACTTCCATTGAGGACAGTCACATTGCCATCTGAAT
CAACGTCCACGATAACTGAATCACCTTCTTTAATCTCATTTGCAAGCATTTTCTCAGCCATGCTGTCCTCTAAAAGTCTCATAATAGCTCTTCTCAGAGG
TCGTGCTCCATAGCTAGGGTTGTATCCCTCGTCAACCACCCTGTCTCTAAACCTCTCTGTCACTTGAAGTTCTATCTCCTTAACTTTCAACCTCTCAAAG
ACCTCCTTAAGCATGATATCAGCTATCTCCTTAACCTCTAACTTAGTGAGCTGACGGAATACAATCATCTCATCCAATCTGTTCAAAAACTCTGGCCTGA
AGTACTGCTTCAATTCTTCTGTCACTAAGCTCTTGATGCGATTGTAACTGCTATCCTTCTCATCAAGGTCGAGATCAAAACCTATACGACGGCCACCTTT
CTCAATTACACTACTTCCAACATTTGATGTCATGATGAGAAGTGTGTTCTTGAAATCTACAGTTCTACCCTTGCTGTCTGTCAATCTTCCATCTTCAAGA
ATTTGAAGCATCATGTTGAAGACATCAGGATGAGCCTTCTCAATCTCATCAAAGAGCACAACTGTATAAGGTCGACGCCTAACAGCTTCAGTCAATTGAC
CACCCTCTGTGTAACCAACATAACCAGGGGGGGATCCAATGAGTTTAGAGACAGTGTGTCTTTCCTTGAACTCACTCATATCAAGCCGGATCATTGCTTC
TTCAGACCCAAAGTAATATGCAGCCAGTGCCTTTGCAGTTCTGATTTCCCACCACAGTTGGACCGGAAAGATGAA
>LE_HBa0015I17_T7_129814 (trimmed for quality and cloning vector)
AGCTTAGTGGCCAACACAAATGAACTCTTCATTTACCACACAGAGACTGTTGAGCTAAAATGTGATCACCTCATCTAAACGGGGATAGGCTCAGGAGCCG
GATCCGAGGGAGTACCACTACTTCCATTGAGGACAGTCACATTGCCATCTGAATCAACGTCCACGATAACTGAATCACCTTCTTTAATCTCATTTGCAAG
CATTTTCTCAGCCATGCTGTCCTCTAAAAGTCTCATAATAGCTCTTCTCAGAGGTCGTGCTCCATAGCTAGGGTTGTATCCCTCGTCAACCACCCTGTCT
CTAAACCTCTCTGTCACTTGAAGTTCTATCTCCTTAACTTTCAACCTCTCAAAGACCTCCTTAAGCATGATATCAGCTATCTCCTTAACCTCTAACTTAG
TGAGCTGACGGAATACAATCATCTCATCCAATCTGTTCAAAAACTCTGGCCTGAAGTACTGCTTCAATTCTTCTGTCACTAAGCTCTTGATGCGATTGTA
ACTGCTATCCTTCTCATCAAGGTCGAGATCAAAACCTATACGACGGCCACCTTTCTCAATTACACTACTTCCAACATTTGATGTCATGATGAGAAGTGTG
TTCTTGAAATCTACAGTTCTACCCTTGCTGTCTGTCAATCTTCCATCTTCAAGAATTTGAAGCATCATGTTGAAGACATCAGGATGAGCCTTCTCAATCT
CATCAAAGAGCACAACTGTATAAGGTCGACGCCTAACAGCTTCAGTCAATTGACCACCCTCTGTGTAACCAACATAACCAGGGGGGGATCCAATGAGTTT
AGAGACAGTGTGTCTTTCCTTGAACTCACTCATATCAAGCCGGATCATTGCTT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.967202
Expected error %
0.5984
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g50920.1 Score 439 E Value 1e-123 Identity 29.90% Align. Length 761bp (89.21%) Frame 1
68418.m06315 ATP-dependent Clp protease ATP-binding subunit / ClpC almost identical to ClpC GI:2921158 from [Arabidopsis thaliana]; contains Pfam profile PF02861: Clp amino terminal domain; contains Pfam profile PF00004: ATPase, AAA family; contains Pfam profile PF02151: UvrB/uvrC motif
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g48870.1 Score 420 E Value 1e-118 Identity 28.30% Align. Length 773bp (90.62%) Frame 1
68416.m05338 ATP-dependent Clp protease ATP-binding subunit (ClpC) identical to AtClpC GI:5360574 from [Arabidopsis thaliana]; contains Pfam profiles PF02861: Clp amino terminal domain and PF02151: UvrB/uvrC motif
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||B35905 Score 471 E Value 1e-131 Identity 31.50% Align. Length 773bp (90.62%) Frame 1
endopeptidase Clp (EC 3.4.21.-) ATP-binding chain cd4B, chloroplast [similarity] - tomato sp|P31542|CLAB_LYCES ATP-dependent clp protease ATP-binding subunit clpA homolog CD4B, chloroplast precursor gb|AAA34161.1| ATP-dependent protease (CD4B)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||A35905 Score 449 E Value 1e-125 Identity 30.40% Align. Length 773bp (90.62%) Frame 1
endopeptidase Clp (EC 3.4.21.-) ATP-binding chain cd4A, chloroplast [similarity] - tomato sp|P31541|CLAA_LYCES ATP-dependent clp protease ATP-binding subunit clpA homolog CD4A, chloroplast precursor gb|AAA34160.1| ATP-dependent protease (CD4A)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0015I17
Clone
Clone name
hba-15i17
LE_HBa0015I17
P015I17
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found