Clone Read LE_HBa0001N12_T7_127700

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001N12_T7_127700
Primer
T7
ID from Seq. Facility
Le-HBa001_N12-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
814 trimmed for vector and quality, 1415 raw
Genbank Accession
DU068314
Sequence
>LE_HBa0001N12_T7_127700 (raw untrimmed)
TTACCTTGGTCCCGGGGTATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATTTTCAAACTTAGGGCTTCTTTTTTGTCAGTTTCTCAAAATCAGCTC
CACAAGCAAAAATAATAATTACTTTTCCTAAAACTAAAAATAGTTATGGAGTGAATGAAAGAAATATCACGCACATGCAACTTTATGTAAATTAAATGCA
ACTACCCATCAATATAAGAACAAGAATCAACAAAATTAAAAAACACAAGGAAAGAAAAATGAAAGCAATTGATACCATAAAATTTGAGCAAAATTACTAA
AAATTAGCACGAACACACAATGAAACTATCAAAAAAGAGGCGGTCGGTTGTGAGCAATCTAGTCTCTTTTGCTCTAGAAGTTGTGGAGGATTTTAGATAT
AGGGAACAAATCCTTAACCGAAATTTAATGACAAAAAATAGCCAAATTGATACCATGTCTATACTCATGCTTTTACTATAGTAGAAAGAGACGGATCGAA
AGCTAAAATGTCTTCGAAATAATAATGGAGGTGAGTACACTTCAAGAGAATTTGAGAGTACTATTCAAACCATGAAATAAGACATGAAAATTCAGTTCCT
AAAACCCCACAACAAAGGAGTAGCCGAGACAATGAATCACACTATTGTTGAGAAGGTGAGAAGCATGCTTAGAGTGGCTAAAATTGCCTAAGTCATTTTG
GGTAAGCAATTTGGACAACATGTTATTTTATCAATGGTATTCCATCAGTTCCATTGGAGCTTTAAATTGTAGAGAGAGTTTTGGGACCCAACATTGAAAT
GTCTTACTCCCACCCTAAAAGGTGGTTCGGTTGCAAAAACTTTTTGCACTTATACCCAAAAGGACCAAAGAACCAGCCTAGATGATTAATTAATTTCCCA
ACCATATTCTTCGGAAATTGAAAAATGAAAAAGTTCCGGGTACACAACTGTGGAAATCTTGTAAAAAAAAAAAGGCAATCAAAAAAAAGAAAAGTAACTC
TTTCGAGAAAACTGAAAGTTTAAACTTTTCTTTTATTCTTTTCGAAGAAAGCCAAAAAAAAAAAGGGGATAAAATTTTTATTCTTTGTACCCTTTTCCTT
CACTTTTCTCCCCCACTCCCTATAACGCTCAACTTTACCAAATATCATTTTTCTAACAACTTACAGAACACCCTTCGCTGTATAATTTTTTGTCCGGGGG
GGGACCAGCTTTGGCGATAAAAATGACTGACGCCCATGGGGAAGCTCCCTTCAACAAATCAACCATAAAAAAACTTCTTCGCTCCTTCGTAAAGGATAAC
AATATAAAAAACAAAAGAGGGCGTAACAGACTACCCCACATANCTCCCCCTTTTCTCCCTTTTGAAAATAATTCATCACACTCTCCACTAATATCAAAAA
GATTGATAAAGCGGG
>LE_HBa0001N12_T7_127700 (trimmed for quality and cloning vector)
AGCTTATTTTCAAACTTAGGGCTTCTTTTTTGTCAGTTTCTCAAAATCAGCTCCACAAGCAAAAATAATAATTACTTTTCCTAAAACTAAAAATAGTTAT
GGAGTGAATGAAAGAAATATCACGCACATGCAACTTTATGTAAATTAAATGCAACTACCCATCAATATAAGAACAAGAATCAACAAAATTAAAAAACACA
AGGAAAGAAAAATGAAAGCAATTGATACCATAAAATTTGAGCAAAATTACTAAAAATTAGCACGAACACACAATGAAACTATCAAAAAAGAGGCGGTCGG
TTGTGAGCAATCTAGTCTCTTTTGCTCTAGAAGTTGTGGAGGATTTTAGATATAGGGAACAAATCCTTAACCGAAATTTAATGACAAAAAATAGCCAAAT
TGATACCATGTCTATACTCATGCTTTTACTATAGTAGAAAGAGACGGATCGAAAGCTAAAATGTCTTCGAAATAATAATGGAGGTGAGTACACTTCAAGA
GAATTTGAGAGTACTATTCAAACCATGAAATAAGACATGAAAATTCAGTTCCTAAAACCCCACAACAAAGGAGTAGCCGAGACAATGAATCACACTATTG
TTGAGAAGGTGAGAAGCATGCTTAGAGTGGCTAAAATTGCCTAAGTCATTTTGGGTAAGCAATTTGGACAACATGTTATTTTATCAATGGTATTCCATCA
GTTCCATTGGAGCTTTAAATTGTAGAGAGAGTTTTGGGACCCAACATTGAAATGTCTTACTCCCACCCTAAAAGGTGGTTCGGTTGCAAAAACTTTTTGC
ACTTATACCCAAAA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.938578
Expected error %
0.9299
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA32025.1| Score 70 E Value 5e-11 Identity 19.90% Align. Length 200bp (24.57%) Frame 1
unnamed protein product [Nicotiana tabacum] sp|P10978|POLX_TOBAC Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Contains: Protease ; Reverse transcriptase ; Endonuclease]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001N12
Clone
Clone name
hba-1n12
LE_HBa0001N12
P001N12
Clone type
BAC
Contamination
none
Estimated length
121,619
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found