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Clone Read LE_HBa0049O17_T7_10305

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0049O17_T7_10305
Primer
T7
ID from Seq. Facility
LE_HBa_049_O17-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
852 trimmed for vector and quality, 905 raw
Genbank Accession
DU029298
Sequence
>LE_HBa0049O17_T7_10305 (raw untrimmed)
TTAACCTGGTACCGCGGGCTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGAGTTTTATTTTGCTTGATATTGGTTTAAAGTTGTTTTCCTAATCCA
ATGTCCTAAAAGAGCAAATGTTCTGTTATTTACATTTTGAGACAATTGTATTATACTGATTGCAGGAGTGCACAATTGATCTGACAGGTCAAACAAATCC
TTCGAGAAGAGCAAGTTTATGGAAAGAGAGACGAGTTTTCTTTGTTACTCCACAAGTGCTGGAGAAGGATATACATTCTGGTGACATTCTGCACTCTAAT
GTGAATACGTTTTTATGTTTTTAGAGTTTTCGTGTTTTCCAGCTATTCGAGGTCTAACCTGCACATGATTTGTGCCTTCATGATAGGAAATAGAACTTAA
CTTCCAGTGAGCTATTTGAATTTCTGGATATCAATGCATAAGCAAGAAAAGAGTCGGGTTATATTTGTATAATTGGATCACTTTAGCGTCACCAACCGTA
ATCTCTCTAATCAGAGATGTTGGAAATATGTCTAAAACTGAATCCATATATTTGTATGTATTTTTTGACATATTCTTGGAGGTATTTTCAGATATTAACT
CTACATTTTCTGCAGAAAAGAAGCTATTTAACTAGCAAGTGAATTTTGTGATTAAACTTTCTTATTTTAAAGAAAAATCAGATGTAAGTATCTAGAGGAC
AGTATTATTTTAAGCACACCAGGTTTAAATAAGAATTATAATAGGTTAATTGCTTGGCCCNAATAGGGTATTAGAGGTATCAGATGGTTAAGATTGTAGA
TGTTAAGTTGTGGTAAATTCAATTATGTGTACTTTATGATAACAACTTTGAGTATAACTTGTGTAGGTTATTGACACTCAAACGTTGTATTTGCTTGAGC
TTTAN
>LE_HBa0049O17_T7_10305 (trimmed for quality and cloning vector)
AGCTTGGAGTTTTATTTTGCTTGATATTGGTTTAAAGTTGTTTTCCTAATCCAATGTCCTAAAAGAGCAAATGTTCTGTTATTTACATTTTGAGACAATT
GTATTATACTGATTGCAGGAGTGCACAATTGATCTGACAGGTCAAACAAATCCTTCGAGAAGAGCAAGTTTATGGAAAGAGAGACGAGTTTTCTTTGTTA
CTCCACAAGTGCTGGAGAAGGATATACATTCTGGTGACATTCTGCACTCTAATGTGAATACGTTTTTATGTTTTTAGAGTTTTCGTGTTTTCCAGCTATT
CGAGGTCTAACCTGCACATGATTTGTGCCTTCATGATAGGAAATAGAACTTAACTTCCAGTGAGCTATTTGAATTTCTGGATATCAATGCATAAGCAAGA
AAAGAGTCGGGTTATATTTGTATAATTGGATCACTTTAGCGTCACCAACCGTAATCTCTCTAATCAGAGATGTTGGAAATATGTCTAAAACTGAATCCAT
ATATTTGTATGTATTTTTTGACATATTCTTGGAGGTATTTTCAGATATTAACTCTACATTTTCTGCAGAAAAGAAGCTATTTAACTAGCAAGTGAATTTT
GTGATTAAACTTTCTTATTTTAAAGAAAAATCAGATGTAAGTATCTAGAGGACAGTATTATTTTAAGCACACCAGGTTTAAATAAGAATTATAATAGGTT
AATTGCTTGGCCCNAATAGGGTATTAGAGGTATCAGATGGTTAAGATTGTAGATGTTAAGTTGTGGTAAATTCAATTATGTGTACTTTATGATAACAACT
TTGAGTATAACTTGTGTAGGTTATTGACACTCAAACGTTGTATTTGCTTGAG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.947091
Expected error %
0.59628
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g35530.1 Score 70 E Value 2e-12 Identity 26.40% Align. Length 128bp (15.02%) Frame 3
68414.m04411 DEAD/DEAH box helicase, putative low similarity to RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana] GI:6102610; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF79368.1| Score 71 E Value 3e-11 Identity 27.60% Align. Length 122bp (14.32%) Frame 3
F15O4.40 [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_174785.1| Score 70 E Value 9e-11 Identity 26.40% Align. Length 128bp (15.02%) Frame 3
DEAD/DEAH box helicase, putative [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0049O17
Clone
Clone name
hba-49o17
LE_HBa0049O17
P049O17
Clone type
BAC
Contamination
none
Estimated length
53,876
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found