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Clone Read LE_HBa0148I02_T7_101379

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0148I02_T7_101379
Primer
T7
ID from Seq. Facility
LE_HBa_148_I02-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
482 trimmed for vector and quality, 832 raw
Genbank Accession
DU091967
Sequence
>LE_HBa0148I02_T7_101379 (raw untrimmed)
CGGGTCCCGGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGGAATACACATTGAGGATCTCCCCTTTGATCATATGATTGAAAAAATAGAC
GTAAATGGAGATCCTTTTATCGATGTAGATGAATTCGGGACATTGTATAAGTCAATCATGGACGAGAAGGACGAAGAGGAGGATATGAGAGAAGCGTTCA
ACGTGTTTGATCAAAATGGTGATGGATATATAACCGTGGAAGAATTAAGGTCCGTTTTGGGATCATTAGGGTTGAAACAAGGTAAAACATTGGAAGATTG
TAAGAGAATGATCATGAAAGTCGACGTGGATGGTGATGGAATGGTTGATTTTAAAGAATTTAGACAAATGATGAAAGGGGGTGGATTTTCATTAACATGA
AATTTATTAGATCAAAAAAAAAGAAAAGGGTATATACCTGTGTATATATATATCCTTTTTGTGTATGGGGTCCTTTGAAATATGCATCCCAGGTCATATT
TCTTTCATCTTGGTCTCATACACTGGCGTTTTTTCTGATGATATAATAATACATTATCTTCGTGCTGCTTATCGTTAACCTTTTCTTAAAGGAAATTGAA
GTTATCTGCCATAAATACTTCGCATATCATTTTTCATTTAATAAAAGATACGGCTTGTTTATTACTATTTGAGTCAATGTTTCGAGTGCCTTTTGCGAAC
TGGCTCTGAACATTGTCCTTTTGAAAATTATTAAGCATCCTTTTATTATCCGCGCCCTTTTTTTCTTTTTATAATTTTGCTTGGAGGAGACCACCTTCTT
ATCTCTTTCCATTGTGAGCTCGCATCCTCGCG
>LE_HBa0148I02_T7_101379 (trimmed for quality and cloning vector)
AGCTTGGGAATACACATTGAGGATCTCCCCTTTGATCATATGATTGAAAAAATAGACGTAAATGGAGATCCTTTTATCGATGTAGATGAATTCGGGACAT
TGTATAAGTCAATCATGGACGAGAAGGACGAAGAGGAGGATATGAGAGAAGCGTTCAACGTGTTTGATCAAAATGGTGATGGATATATAACCGTGGAAGA
ATTAAGGTCCGTTTTGGGATCATTAGGGTTGAAACAAGGTAAAACATTGGAAGATTGTAAGAGAATGATCATGAAAGTCGACGTGGATGGTGATGGAATG
GTTGATTTTAAAGAATTTAGACAAATGATGAAAGGGGGTGGATTTTCATTAACATGAAATTTATTAGATCAAAAAAAAAGAAAAGGGTATATACCTGTGT
ATATATATATCCTTTTTGTGTATGGGGTCCTTTGAAATATGCATCCCAGGTCATATTTCTTTCATCTTGGTCTCATACACTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.928349
Expected error %
1.33482
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g07490.1 Score 148 E Value 1e-36 Identity 21.30% Align. Length 347bp (71.99%) Frame 2
68416.m00893 calcium-binding protein, putative similar to calcium-binding protein GI:6580549 from [Lotus japonicus]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g05990.1 Score 145 E Value 8e-36 Identity 21.30% Align. Length 347bp (71.99%) Frame 2
68414.m00627 calcium-binding protein, putative strong similarity to calcium-binding protein [Lotus japonicus] GI:18413495; contains INTERPRO:IPR002048 calcium-binding EF-hand domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB63264.3| Score 150 E Value 1e-35 Identity 21.30% Align. Length 347bp (71.99%) Frame 2
calcium-binding protein [Lotus corniculatus var. japonicus]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF02168.1| Score 148 E Value 5e-35 Identity 21.30% Align. Length 347bp (71.99%) Frame 2
putative calmodulin [Arabidopsis thaliana] ref|NP_187405.1| calcium-binding protein, putative [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0148I02
Clone
Clone name
hba-148i2
LE_HBa0148I02
P148I02
Clone type
BAC
Contamination
none
Estimated length
79,001
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found