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Clone hba-115k16

Clone and library  
Clone
Clone name
hba-115k16
LE_HBa0115K16
C10HBa0115K16
P115K16
Clone type
BAC
Contamination
none
Estimated length
88,437
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg1375 : [coherent], 10 members:
C10HBa0029I02, LE_HBa0042B06, LE_HBa0104H22, C10HBa0115K16, LE_HBa0154L01, C10HBa0238G16, LE_HBa0239H21, LE_HBa0246C08, C10HBa0304I01, LE_HBa0323M08
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T0590Tomato-EXPEN 2000plate 16A9
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 10
FISH map 
View on FISH map chromosome 10
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
653-03 1CompositeFluorescencePhase12.7%
653-05 1CompositeFluorescencePhase12.6%
653-06 1CompositeFluorescencePhase12.2%
653-08 1CompositeFluorescencePhase10.1%
653-11 1CompositeFluorescencePhase11.8%
653-12 1CompositeFluorescencePhase11.7%
653-13 1CompositeFluorescencePhase12.3%
653-21 1CompositeFluorescencePhase11.3%
653-23 1CompositeFluorescencePhase12.5%
653-24 1CompositeFluorescencePhase10.0%
653-25 1CompositeFluorescencePhase8.5%
653-26 1CompositeFluorescencePhase11.7%
653-37 1CompositeFluorescencePhase6.8%
653-48 1CompositeFluorescencePhase13.3%
653-49 1CompositeFluorescencePhase7.4%
653-51 1CompositeFluorescencePhase7.8%
Mean±sd10.8%±2.1%

Given an estimated length of 10.1µm for chromosome 10Q and the mean percentage above, this BAC is approximately 1.1µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 10 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 10
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
163,868 bp
C10HBa0115K16.1
AC193780.1 (GenBank) 		[View]
End Sequences
Warnings
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
  • sequence length 163,868 is 85.3% different from estimated length 88,437
 FTP
Chromosome 10 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (20)
2068, 2128, 2301, 2301, 2779, 2979, 2979, 3265, 3410, 3644, 3844, 4088, 4088, 5061, 5913, 5913, 6378, 6550, 9374, 9374
HindIII in silico (50)
1002, 1008, 1010, 1014, 1052, 1117, 1117, 1151, 1184, 1250, 1363, 1469, 1480, 1480, 1502, 1714, 1769, 1776, 1834, 2023, 2039, 2106, 2113, 2113, 2241, 2301, 2309, 2320, 2744, 2800, 2954, 2986, 3030, 3287, 3420, 3638, 3704, 3852, 4095, 4151, 4859, 5547, 5567, 5686, 6214, 6257, 6412, 6562, 9279, 9517
Fragment Lengths Match Score
0.63
Annotations  
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LE_HBa0115K16 has no browsable sequence annotations
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End Sequences

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Map
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IL Bin Mapping
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 Verification

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