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SC Spread ID	Images			Distance from centromere (percentage of arm length)
221-08 1	Composite	Fluorescence	Phase	97.1%
222-10 1	Composite	Fluorescence	Phase	95.5%
221-12 1	Composite	Fluorescence	Phase	98.7%
222-12 1	Composite	Fluorescence	Phase	100.0%
222-13 1	Composite	Fluorescence	Phase	96.8%
222-16 1	Composite	Fluorescence	Phase	98.7%
221-16 1	Composite	Fluorescence	Phase	100.0%
221-17 1	Composite	Fluorescence	Phase	96.9%
222-17 1	Composite	Fluorescence	Phase	96.9%
221-18 1	Composite	Fluorescence	Phase	100.0%
222-20 1	Composite	Fluorescence	Phase	97.6%
221-21 1	Composite	Fluorescence	Phase	97.9%
221-24 1	Composite	Fluorescence	Phase	100.0%
221-25 1	Composite	Fluorescence	Phase	97.5%
221-26 1	Composite	Fluorescence	Phase	96.9%
Mean±sd				98.0%±1.4%

Given an estimated length of 7.7µm for chromosome 5Q and the mean percentage above, this BAC is approximately 7.5µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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