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SC Spread ID	Images			Distance from centromere (percentage of arm length)
695-05 1	Composite	Fluorescence	Phase	9.5%
695-07 1	Composite	Fluorescence	Phase	5.8%
695-08 1	Composite	Fluorescence	Phase	8.0%
695-12 1	Composite	Fluorescence	Phase	7.4%
695-14 1	Composite	Fluorescence	Phase	6.6%
695-25 1	Composite	Fluorescence	Phase	8.5%
695-28a 1	Composite	Fluorescence	Phase	7.2%
695-28b 1	Composite	Fluorescence	Phase	6.3%
695-29 1	Composite	Fluorescence	Phase	7.2%
695-39 1	Composite	Fluorescence	Phase	7.8%
Mean±sd				7.4%±1.1%

Given an estimated length of 7.3µm for chromosome 12Q and the mean percentage above, this BAC is approximately 0.5µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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