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SC Spread ID	Images			Distance from centromere (percentage of arm length)
632-03 1	Composite	Fluorescence	Phase	13.7%
645-07 1	Composite	Fluorescence	Phase	17.0%
632-12 1	Composite	Fluorescence	Phase	16.8%
645-14 1	Composite	Fluorescence	Phase	10.9%
645-17 1	Composite	Fluorescence	Phase	15.2%
645-18 1	Composite	Fluorescence	Phase	15.0%
632-22 1	Composite	Fluorescence	Phase	19.4%
645-24 1	Composite	Fluorescence	Phase	12.8%
632-24 1	Composite	Fluorescence	Phase	14.3%
632-30 1	Composite	Fluorescence	Phase	18.7%
632-31 1	Composite	Fluorescence	Phase	17.5%
632-32 1	Composite	Fluorescence	Phase	19.8%
632-34 1	Composite	Fluorescence	Phase	16.0%
645-46 1	Composite	Fluorescence	Phase	18.6%
Mean±sd				16.1%±2.6%

Given an estimated length of 7.3µm for chromosome 12Q and the mean percentage above, this BAC is approximately 1.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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