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SC Spread ID	Images			Distance from centromere (percentage of arm length)
299-03 1	Composite	Fluorescence	Phase	65.9%
299-09 1	Composite	Fluorescence	Phase	70.0%
299-16 1	Composite	Fluorescence	Phase	71.1%
299-19 1	Composite	Fluorescence	Phase	71.2%
299-20 1	Composite	Fluorescence	Phase	67.5%
299-21 1	Composite	Fluorescence	Phase	67.2%
299-22 1	Composite	Fluorescence	Phase	71.8%
299-23a 1	Composite	Fluorescence	Phase	70.7%
299-23b 1	Composite	Fluorescence	Phase	70.0%
299-24 1	Composite	Fluorescence	Phase	71.1%
299-25 1	Composite	Fluorescence	Phase	67.2%
Mean±sd				69.4%±2.1%

Given an estimated length of 18.9µm for chromosome 2Q and the mean percentage above, this BAC is approximately 13.1µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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