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SC Spread ID	Images			Distance from centromere (percentage of arm length)
293-02 1	Composite	Fluorescence	Phase	23.9%
293-04 1	Composite	Fluorescence	Phase	25.2%
293-06 1	Composite	Fluorescence	Phase	23.5%
293-11 1	Composite	Fluorescence	Phase	23.9%
293-20 1	Composite	Fluorescence	Phase	23.4%
293-26 1	Composite	Fluorescence	Phase	24.8%
293-28 1	Composite	Fluorescence	Phase	21.9%
293-31 1	Composite	Fluorescence	Phase	24.5%
293-32 1	Composite	Fluorescence	Phase	25.3%
293-35a 1	Composite	Fluorescence	Phase	21.3%
293-35b 1	Composite	Fluorescence	Phase	22.4%
293-38 1	Composite	Fluorescence	Phase	24.6%
293-39 1	Composite	Fluorescence	Phase	24.9%
293-40 1	Composite	Fluorescence	Phase	24.4%
Mean±sd				23.9%±1.2%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 4.8µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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