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SC Spread ID	Images			Distance from centromere (percentage of arm length)
771-06 1	Composite	Fluorescence	Phase	14.4%
771-08 1	Composite	Fluorescence	Phase	16.2%
771-09 1	Composite	Fluorescence	Phase	13.8%
771-10 1	Composite	Fluorescence	Phase	15.1%
771-22 1	Composite	Fluorescence	Phase	17.4%
771-27 1	Composite	Fluorecence	Phase	17.7%
771-38 1	Composite	Fluorescence	Phase	16.3%
771-40 1	Composite	Fluorescence	Phase	16.3%
771-43 1	Composite	Fluorescence	Phase	14.2%
771-45 1	Composite	Fluorescence	Phase	14.3%
771-49 1	Composite	Fluorescence	Phase	15.7%
771-50 1	Composite	Fluorescence	Phase	15.8%
Mean±sd				15.6%±1.3%

Given an estimated length of 15.7µm for chromosome 3Q and the mean percentage above, this BAC is approximately 2.4µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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