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SC Spread ID	Images			Distance from centromere (percentage of arm length)
659-02 1	Composite	Fluorescence	Phase	16.4%
659-14 1	Composite	Fluorescence	Phase	17.9%
659-18 1	Composite	Fluorescence	Phase	15.8%
659-45 1	Composite	Fluorescence	Phase	15.5%
659-46 1	Composite	Fluorescence	Phase	14.4%
659-48 1	Composite	Fluorescence	Phase	17.6%
659-51 1	Composite	Fluorescence	Phase	15.8%
659-60 1	Composite	Fluorescence	Phase	18.4%
Mean±sd				16.5%±1.4%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 2.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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