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SC Spread ID	Images			Distance from centromere (percentage of arm length)
663-09 1	Composite	Fluorescence	Phase	71.5%
663-10 1	Composite	Fluorescence	Phase	67.6%
663-23 1	Composite	Fluorescence	Phase	67.9%
663-24 1	Composite	Fluorescence	Phase	69.6%
663-33 1	Composite	Fluorescence	Phase	71.7%
663-35 1	Composite	Fluorescence	Phase	68.9%
663-41 1	Composite	Fluorescence	Phase	68.6%
663-46 1	Composite	Fluorescence	Phase	68.8%
663-47 1	Composite	Fluorescence	Phase	66.6%
663-49 1	Composite	Fluorescence	Phase	67.3%
663-50a 1	Composite	Fluorescence	Phase	69.4%
663-50b 1	Composite	Fluorescence	Phase	69.3%
663-54 1	Composite	Fluorescence	Phase	66.5%
Mean±sd				68.7%±1.6%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 13.8µm±0.3µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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