Clone Read LE_HBa0004E13_SP6_984

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0004E13_SP6_984
Primer
SP6
ID from Seq. Facility
LE_Hba_004_E13-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
742 trimmed for vector and quality, 1201 raw
Sequence
>LE_HBa0004E13_SP6_984 (raw untrimmed)
GGCAGCTCTTAATGTACACCAAGCAATATTTTACAATAATGTTTGAAAAAAGGAAAGTTTTCATGGACAAATGGCTCCTTAGCAAGCACTAGCCCCATCA
TTTCCGCACGTCTTCATGTGTGCCCTTTTTATATTCTTATTATATATGGAGTATCATTGGATTTCAAAGACAATTTAGAGCCCACCCATCCTTGACATAT
ATCAACACTAAACACTATAGAGCCAAAAAAGCAGTGATGCTGCTTTCAATGCTTGGCTTGCGGGTCATTGCCCACTGTATGACTGAACTGAGGGGACGTT
CTGAAAGAGAAATAAAAGAGGGACAGTAGTGGGCATATGTAGAGAACGAAAGAGAAAAGGGTTGGGCTAAATTTTTGTGTTTTGAAGACAAAAAGTTTAA
AGAATCTCTTTCTAGTTGCTATGGAACAATTAGAAGATTCTCTAGAACAAATGCGGTGTTCTGCTAAATTTTAATATGTTAGTTGTGCTACTAGGCATAT
TTAAAGGGGAGAATTGGTTATAAAACGAACCTGCTACCGTCATGTATATATAATGATAATATGGTTAAGAATTCAAATGTTTCTCTTATTTGTTTTCTTT
GGATAAGAAGAAAGATCCTATACTATATTGGAGGGGTTATCTGCTTAGCAGAAACTTATGGAAGCTCACGTTGTGTTATTGACTTATTTCACTGTTACTA
TCATCTTCATGCTCTATTATAATTTTGTATCAAGTACAACCAAAATGCTTCTTGTCTTTTATATTAATTCAACTTCAAGTGTTTATATAGGTCTGAGATT
CATGGAAAAAATGAGCTGCCACCGCTGTCAACCAGATGCAAGGATGCCAGATGACAACTTCATAGCTGACTTGCACACTCAGGGTCATCACCCTGATCAA
GAAGATATGGGGGGGTTGGAGGTGTAGCTGGGTGCTTGGATACATTAAAAAACTTCAAAAGGGCGGGAAAGTATTTTTTTTTACCGGCCAAATATTCATT
TCTGTTTTTTGGTTCCTATTTACCAAACCCCCTTTTCCCAAAAGGAACTTCCCAAGGAAAAAGGAATGGCCCATTGGGGGTCATTTTTTTTCCCAAAAAT
CGTACCAAAATTAAATTTCCCCCCCCGCCCCGGAGAAAGAGGTAAACCAAGAAGTTCAAATTTTGGTTGACTTTTTTCCCAAAAAACCCTAAAAAAATTA
A
>LE_HBa0004E13_SP6_984 (trimmed for quality and cloning vector)
GCAATATTTTACAATAATGTTTGAAAAAAGGAAAGTTTTCATGGACAAATGGCTCCTTAGCAAGCACTAGCCCCATCATTTCCGCACGTCTTCATGTGTG
CCCTTTTTATATTCTTATTATATATGGAGTATCATTGGATTTCAAAGACAATTTAGAGCCCACCCATCCTTGACATATATCAACACTAAACACTATAGAG
CCAAAAAAGCAGTGATGCTGCTTTCAATGCTTGGCTTGCGGGTCATTGCCCACTGTATGACTGAACTGAGGGGACGTTCTGAAAGAGAAATAAAAGAGGG
ACAGTAGTGGGCATATGTAGAGAACGAAAGAGAAAAGGGTTGGGCTAAATTTTTGTGTTTTGAAGACAAAAAGTTTAAAGAATCTCTTTCTAGTTGCTAT
GGAACAATTAGAAGATTCTCTAGAACAAATGCGGTGTTCTGCTAAATTTTAATATGTTAGTTGTGCTACTAGGCATATTTAAAGGGGAGAATTGGTTATA
AAACGAACCTGCTACCGTCATGTATATATAATGATAATATGGTTAAGAATTCAAATGTTTCTCTTATTTGTTTTCTTTGGATAAGAAGAAAGATCCTATA
CTATATTGGAGGGGTTATCTGCTTAGCAGAAACTTATGGAAGCTCACGTTGTGTTATTGACTTATTTCACTGTTACTATCATCTTCATGCTCTATTATAA
TTTTGTATCAAGTACAACCAAAATGCTTCTTGTCTTTTATAT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.958411
Expected error %
0.241063
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 98 E Value 5e-22 Identity 92.80% Align. Length 68bp (9.16%) Frame 1
Nicotiana tabacum plastid, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0004E13
Clone
Clone name
hba-4e13
LE_HBa0004E13
P004E13
Clone type
BAC
Contamination
none
Estimated length
51,584
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found