Clone Read LE_HBa0150M11_SP6_90836

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0150M11_SP6_90836
Primer
SP6
ID from Seq. Facility
LE_HBa_150_M11-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
561 trimmed for vector and quality, 841 raw
Genbank Accession
DU058914
Sequence
>LE_HBa0150M11_SP6_90836 (raw untrimmed)
TTCGGGTTGTACCATTGTAACTCCCATTTTCTCTGCAGCCTTTTTCGCAATAACCATTCGTTCCTTGGCGTCCTTCAAGTAAGCTTGAATTGTTCCGAAT
ATCAAAGGCTGATCATCTTTGTGATACTTAATTGCTGCTGAATACGCAAAATAATCAATTGCAGGTTGAATGGTTGTGTCTTCCGCATCAATTAGTAATG
GAACTTCATGTTCCAAACATTTCTCGCAAATTTTTCTAAGTCTTTCATGAGCTAATTGAAGATCACGCTCTTCCTCTACCGATAGAGGCTCTGGTTTGCT
ACAAGTGTGATAAACAGGGCTCGATTCGGCGAAAAGGGGAAGCGACTCTCGCTTCCATGGAAGATTAAATGATGGATCTTTCTGTTCCCATCTTAACAAA
TCACTCATTCTTTTGAGAAGCCTTGGGGTACAAATAGCAGTTATCTTTGCTACCACAAAACTTGCCTGTTTCACCCCAAAAAAAGAAAAATTTAAAAAAA
TGAAATTCATAAATCCAAGAATATTGGAAACACAAATAGAATATTATTTGAAATTCTTACAGAAGATTCGGGAAGTGATTTGGTTGATTCAATCGTTTGA
ATAAACGCCTCAGTACTCTGTTCACCAACATTCATTATCTGTGGCTTGTTCTACCCCATAATCAACCATGGTTTTTAACCCAGAAATCTCTATATAATTA
ATAACGGTCCTTTCTTCCTTTCTTTAATTCTTTTGTCTTGCCAATATTGTCATTAAATGTTATTTTTATATACCCATAAATTTTCCCCTCCTAAATTAAG
TCATTTTCCTTAATTTTAATAATTTTATTACTCCCATCTCT
>LE_HBa0150M11_SP6_90836 (trimmed for quality and cloning vector)
TTTCTCTGCAGCCTTTTTCGCAATAACCATTCGTTCCTTGGCGTCCTTCAAGTAAGCTTGAATTGTTCCGAATATCAAAGGCTGATCATCTTTGTGATAC
TTAATTGCTGCTGAATACGCAAAATAATCAATTGCAGGTTGAATGGTTGTGTCTTCCGCATCAATTAGTAATGGAACTTCATGTTCCAAACATTTCTCGC
AAATTTTTCTAAGTCTTTCATGAGCTAATTGAAGATCACGCTCTTCCTCTACCGATAGAGGCTCTGGTTTGCTACAAGTGTGATAAACAGGGCTCGATTC
GGCGAAAAGGGGAAGCGACTCTCGCTTCCATGGAAGATTAAATGATGGATCTTTCTGTTCCCATCTTAACAAATCACTCATTCTTTTGAGAAGCCTTGGG
GTACAAATAGCAGTTATCTTTGCTACCACAAAACTTGCCTGTTTCACCCCAAAAAAAGAAAAATTTAAAAAAATGAAATTCATAAATCCAAGAATATTGG
AAACACAAATAGAATATTATTTGAAATTCTTACAGAAGATTCGGGAAGTGATTTGGTTGAT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.955046
Expected error %
0.216863
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g30775.1 Score 189 E Value 7e-49 Identity 21.10% Align. Length 434bp (77.36%) Frame 2
68416.m03933 proline oxidase, mitochondrial / osmotic stress-responsive proline dehydrogenase (POX) (PRO1) (ERD5) nearly identical to SP|P92983 Proline oxidase, mitochondrial precursor (EC 1.5.3.-) (Osmotic stress- induced proline dehydrogenase) [Arabidopsis thaliana]; identical to cDNA proline oxidase precursor GI:1817543
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g38710.1 Score 187 E Value 3e-48 Identity 20.50% Align. Length 434bp (77.36%) Frame 2
68418.m04682 proline oxidase, putative / osmotic stress-responsive proline dehydrogenase, putative similar to proline oxidase, mitochondrial precursor (Osmotic stress- induced proline dehydrogenase) [Arabidopsis thaliana] SWISS-PROT:P92983
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT57675.1| Score 267 E Value 1e-70 Identity 29.70% Align. Length 434bp (77.36%) Frame 2
proline oxidase/dehydrogenase 2 [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT57674.1| Score 266 E Value 2e-70 Identity 29.70% Align. Length 434bp (77.36%) Frame 2
proline oxidase/dehydrogenase 1 [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0150M11
Clone
Clone name
hba-150m11
LE_HBa0150M11
C02HBa0150M11
P150M11
Clone type
BAC
Contamination
none
Estimated length
172,243
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found