Clone Read LE_HBa0150M08_SP6_90830

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0150M08_SP6_90830
Primer
SP6
ID from Seq. Facility
LE_HBa_150_M08-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
684 trimmed for vector and quality, 825 raw
Genbank Accession
DU058909
Sequence
>LE_HBa0150M08_SP6_90830 (raw untrimmed)
TCGTTGGTCCAAGAGCCTCAAGCCTAGTTAACATATTCCACCCTGGTTTTTGTGAGCATGGCAATGTTAACATGATGGAGCTCCCGAAAGCTGAGACCTT
TATCCTCCTTTTCCTGACAAACTTTCTGCCAAGACATCCACTTTATTCCTCGATTTTCTCTCGTTCCCGATTTCCACAAAAATCCATTCATAGCTCGCTC
AACAGGTTCACATATATTACTGGGAAGTACGAACATATTCATCACATAGGTTGGAATTGATTGAACCACTGTTTTGAACAATACTTCTCGCCTTGCTCGA
GAAAGACTAGTATACTTCCAACTGTTTATCTTTGCAATTACTCTATTATGAATATAAGCAAAGACAAATTTTTTGCTTCTTCCTATTAGTGCCGGCAATG
CAAGATACTTTACAGGAGAATTCACCTCCTTGACATAAAGGATATCTACGATTTCTTTTTTCACACTTTCATTTGTATTAGGACTGAAGAAAATATCATA
TTTTTGAAAATTTACCTGTTGACCAGAGGCCGCCTGATAAATCTCCAAACATAGCTTGATAGTTTTTGCCTCCTCCTTTGCTCCTTTAAAAAAATATAAA
GCATCATCTGCAAAAAATAGGTGTGAAATAGATGGTGCTTTATTGCAAATTTTGACACCATGAATTCGACTCTGAATAGACATTCGTTTCAACAATGTCT
GATAAACCTTCTACAACTATAAAAAATAGAAAAGGTGATATTGGATCACCTGGCCTCTAGCCTCTCCTGGGAGAAATATTTTCGAACTGGTTTGCCATCA
TGAACTAAATTCACAAATAACGGCC
>LE_HBa0150M08_SP6_90830 (trimmed for quality and cloning vector)
AGCCTCAAGCCTAGTTAACATATTCCACCCTGGTTTTTGTGAGCATGGCAATGTTAACATGATGGAGCTCCCGAAAGCTGAGACCTTTATCCTCCTTTTC
CTGACAAACTTTCTGCCAAGACATCCACTTTATTCCTCGATTTTCTCTCGTTCCCGATTTCCACAAAAATCCATTCATAGCTCGCTCAACAGGTTCACAT
ATATTACTGGGAAGTACGAACATATTCATCACATAGGTTGGAATTGATTGAACCACTGTTTTGAACAATACTTCTCGCCTTGCTCGAGAAAGACTAGTAT
ACTTCCAACTGTTTATCTTTGCAATTACTCTATTATGAATATAAGCAAAGACAAATTTTTTGCTTCTTCCTATTAGTGCCGGCAATGCAAGATACTTTAC
AGGAGAATTCACCTCCTTGACATAAAGGATATCTACGATTTCTTTTTTCACACTTTCATTTGTATTAGGACTGAAGAAAATATCATATTTTTGAAAATTT
ACCTGTTGACCAGAGGCCGCCTGATAAATCTCCAAACATAGCTTGATAGTTTTTGCCTCCTCCTTTGCTCCTTTAAAAAAATATAAAGCATCATCTGCAA
AAAATAGGTGTGAAATAGATGGTGCTTTATTGCAAATTTTGACACCATGAATTCGACTCTGAATAGACATTCGTTTCAACAATG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.954936
Expected error %
0.31472
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g36905.1 Score 71 E Value 6e-13 Identity 10.80% Align. Length 350bp (51.17%) Frame 3
68418.m04423 RNase H domain-containing protein low similarity to reverse transcriptase [Arabidopsis thaliana] GI:976278; contains Pfam profile PF00075: RNase H
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g24255.1 Score 69 E Value 3e-12 Identity 9.30% Align. Length 461bp (67.40%) Frame 3
68416.m03045 expressed protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP53315.1| Score 171 E Value 2e-41 Identity 12.50% Align. Length 647bp (94.59%) Frame 3
putative reverse transcriptase [Oryza sativa (japonica cultivar-group)] ref|NP_921028.1| putative reverse transcriptase [Oryza sativa (japonica cultivar-group)] gb|AAM18736.1| putative reverse transcriptase [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_475290.1| Score 171 E Value 2e-41 Identity 12.50% Align. Length 647bp (94.59%) Frame 3
hypothetical protein [Oryza sativa (japonica cultivar-group)] gb|AAT58873.1| hypothetical protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0150M08
Clone
Clone name
hba-150m8
LE_HBa0150M08
P150M08
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found