Clone Read LE_HBa0120P21_T7_87929

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0120P21_T7_87929
Primer
T7
ID from Seq. Facility
LE_HBa_120_P21-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
376 trimmed for vector and quality, 820 raw
Sequence
>LE_HBa0120P21_T7_87929 (raw untrimmed)
CTCTGATCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTAGCGGCTCAAAGTAGACAAAAAGAGTATGCAGATCGAAAGGTTAGGGACT
TGGAGTTAATGGAGGGTGAGCAAGTCTTACTGAAGGTTTCGCCCATGAAAGGAGTGATGCGGTTTGTTAAGCGAGGTAAGCTAAGCCCAAGGTATATTGG
TCCATTTGAAGTTCTGAAGCGCGTGGGGGAGGTAGCTTATGAATTAGCCTTGCCCCCAGGACTGTCCGGAGTGCACCCAGTATTTTATGTTTCTATGTTG
AAAAGATACCATGGGGATGGAAACTACATCATTCATTGGGATTTAGTTCTGCTTGATGAGAATCTCTCCTATGAGGAGGAGCCTGTTGCTATTTTATATA
GAGAATTCCGCAAGTTGAGGTCAAGGGAGATTGTATCCATCTATGTTCAATGAAAGAATCTACTGGTTAATTAATCCACTTGGGAGAATGAGGCTTTATT
TCCACATATATTTCTTCGGTTGTTTTCTTATTCTTGGTCTTTTTTTTCCCGTTTACCTTATTTCTTTGTAATCTTTTTGGGTCCAACTATTCGTTTATAG
GTTATATAATTGTTACGTTGATTTACGGTTTGTCTTGTTAATCTTTCTAGTCATTTGTTTGTTCTAATTGCTTTTCTGCTATATTTTATTCTTGTGTGTT
TTGTTGCTAGCTGTTAGTAGTTTGTGGATAATATATATTAACTTTATATACCATCCTTTTCCTGATCCTAATTTTATAAGTTTCTACTTGTCAGTCCTTT
TTATTATTTTTTCTTCTGAT
>LE_HBa0120P21_T7_87929 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTAGCGGCTCAAAGTAGACAAAAAGAGTATGCAGATCGAAAGGTTAGGGACTTGGAGTTAATGGAGGGT
GAGCAAGTCTTACTGAAGGTTTCGCCCATGAAAGGAGTGATGCGGTTTGTTAAGCGAGGTAAGCTAAGCCCAAGGTATATTGGTCCATTTGAAGTTCTGA
AGCGCGTGGGGGAGGTAGCTTATGAATTAGCCTTGCCCCCAGGACTGTCCGGAGTGCACCCAGTATTTTATGTTTCTATGTTGAAAAGATACCATGGGGA
TGGAAACTACATCATTCATTGGGATTTAGTTCTGCTTGATGAGAATCTCTCCTATGAGGAGGAGCCTGTTGCTATT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.957739
Expected error %
0.0509337
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 66 E Value 3e-12 Identity 100.00% Align. Length 32bp (8.51%) Frame 2
Cloning vector pMAMneoBlue
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02437.1:4662-4674-49 Score 66 E Value 3e-12 Identity 100.00% Align. Length 32bp (8.51%) Frame 2
Cloning vector pT3/T7-LUC
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT66771.1| Score 150 E Value 1e-35 Identity 20.50% Align. Length 350bp (93.09%) Frame 3
putative polyprotein [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAV31169.1| Score 150 E Value 1e-35 Identity 20.50% Align. Length 350bp (93.09%) Frame 3
putative polyprotein [Solanum tuberosum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0120P21
Clone
Clone name
hba-120p21
LE_HBa0120P21
P120P21
Clone type
BAC
Contamination
none
Estimated length
125,297
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found